A Screening Method to Identify Essential Yeast Genes for Responses Towards Spermine

Mustapha Aouida*, Dindial Ramotar

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

We exploited the yeast DAmP mutant collection to identify essential genes that play a role in polyamine resistance. Herein, we described in details the methodology to obtain these genes. This approach is applicable for screening many nontoxic and toxic drugs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages363-369
Number of pages7
DOIs
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2377
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Essential gene
  • Polyamine resistance
  • Spermine
  • Yeast

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