TY - JOUR
T1 - Acid-induced structural changes in human rhinovirus 14
T2 - Possible role in uncoating
AU - Giranda, Vincent L.
AU - Heinz, Beverly A.
AU - Oliveira, Marcos A.
AU - Minor, Iwona
AU - Kim, Kyung Hyun
AU - Kolatkar, Prasanna R.
AU - Rossmann, Michael G.
AU - Rueckert, Roland R.
PY - 1992
Y1 - 1992
N2 - X-ray diffraction data were collected from human rhinovirus 14 crystals a few minutes after exposure to acid vapor and prior to excessive crystalline disorder. Conformational changes occurred (i) in the GH loop of viral protein (VP) 1, (ii) at the ion binding site on the outer surface of the pentamer center, and (iii) in VP3 and VP4 on the virion's interior in the vicinity of the fivefold axis. Amino acid substitutions in mutants resistant to low pH, or to drugs that inhibit uncoating, were concentrated in the vicinity of the GH loop. It is proposed that the acid-induced changes reflect processes that trigger uncoating.
AB - X-ray diffraction data were collected from human rhinovirus 14 crystals a few minutes after exposure to acid vapor and prior to excessive crystalline disorder. Conformational changes occurred (i) in the GH loop of viral protein (VP) 1, (ii) at the ion binding site on the outer surface of the pentamer center, and (iii) in VP3 and VP4 on the virion's interior in the vicinity of the fivefold axis. Amino acid substitutions in mutants resistant to low pH, or to drugs that inhibit uncoating, were concentrated in the vicinity of the GH loop. It is proposed that the acid-induced changes reflect processes that trigger uncoating.
UR - http://www.scopus.com/inward/record.url?scp=0026476473&partnerID=8YFLogxK
U2 - 10.1073/pnas.89.21.10213
DO - 10.1073/pnas.89.21.10213
M3 - Article
C2 - 1332036
AN - SCOPUS:0026476473
SN - 0027-8424
VL - 89
SP - 10213
EP - 10217
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 21
ER -