An improved commercial method for determination of nicotine in tobacco

Md Wasim Aktar; M. Paramasivam; Dwaipayan Sengupta

doi:10.1080/10496475.2010.481922

An improved commercial method for determination of nicotine in tobacco

Md Wasim Aktar, M. Paramasivam, Dwaipayan Sengupta^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A high-pressure thin-layer chromatography (HPTLC) method for quantitative analysis of nicotine in Nicotiana sp. was developed using a methanol extract of leaves and stems and TLC plates (silica gel 60 GF₂₅₄) with spot visualization under ultraviolet (UV) light. Scanning at 235 nm in the absorption-reflection mode produced linear calibration curves in the range of 2 to 25 g, with a correlation coefficient of 0.991. The average recovery rate was 95% (CV % 1.35). From the present study, the lower limit of detection was 0.08 g spot-1 for the nicotine. The validity of the method was confirmed by comparing the UV spectra of the tobacco plant samples with standards within the same Rf window.

Original language	English
Pages (from-to)	36-40
Number of pages	5
Journal	Journal of Herbs, Spices and Medicinal Plants
Volume	16
Issue number	1
DOIs	https://doi.org/10.1080/10496475.2010.481922
Publication status	Published - Jan 2010
Externally published	Yes

Keywords

Analysis
Formulation
HPTLC

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10.1080/10496475.2010.481922

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abstract = "A high-pressure thin-layer chromatography (HPTLC) method for quantitative analysis of nicotine in Nicotiana sp. was developed using a methanol extract of leaves and stems and TLC plates (silica gel 60 GF254) with spot visualization under ultraviolet (UV) light. Scanning at 235 nm in the absorption-reflection mode produced linear calibration curves in the range of 2 to 25 g, with a correlation coefficient of 0.991. The average recovery rate was 95% (CV % 1.35). From the present study, the lower limit of detection was 0.08 g spot-1 for the nicotine. The validity of the method was confirmed by comparing the UV spectra of the tobacco plant samples with standards within the same Rf window.",

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author = "Aktar, {Md Wasim} and M. Paramasivam and Dwaipayan Sengupta",

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T1 - An improved commercial method for determination of nicotine in tobacco

AU - Aktar, Md Wasim

AU - Paramasivam, M.

AU - Sengupta, Dwaipayan

PY - 2010/1

Y1 - 2010/1

N2 - A high-pressure thin-layer chromatography (HPTLC) method for quantitative analysis of nicotine in Nicotiana sp. was developed using a methanol extract of leaves and stems and TLC plates (silica gel 60 GF254) with spot visualization under ultraviolet (UV) light. Scanning at 235 nm in the absorption-reflection mode produced linear calibration curves in the range of 2 to 25 g, with a correlation coefficient of 0.991. The average recovery rate was 95% (CV % 1.35). From the present study, the lower limit of detection was 0.08 g spot-1 for the nicotine. The validity of the method was confirmed by comparing the UV spectra of the tobacco plant samples with standards within the same Rf window.

AB - A high-pressure thin-layer chromatography (HPTLC) method for quantitative analysis of nicotine in Nicotiana sp. was developed using a methanol extract of leaves and stems and TLC plates (silica gel 60 GF254) with spot visualization under ultraviolet (UV) light. Scanning at 235 nm in the absorption-reflection mode produced linear calibration curves in the range of 2 to 25 g, with a correlation coefficient of 0.991. The average recovery rate was 95% (CV % 1.35). From the present study, the lower limit of detection was 0.08 g spot-1 for the nicotine. The validity of the method was confirmed by comparing the UV spectra of the tobacco plant samples with standards within the same Rf window.

KW - Analysis

KW - Formulation

KW - HPTLC

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M3 - Article

AN - SCOPUS:77952639755

SN - 1049-6475

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SP - 36

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JO - Journal of Herbs, Spices and Medicinal Plants

JF - Journal of Herbs, Spices and Medicinal Plants

IS - 1

ER -

An improved commercial method for determination of nicotine in tobacco

Abstract

Keywords

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