An optimized protocol for the preparation of blood immune cells for transmission electron microscopy

Nimshitha Pavathuparambil Abdul Manaph; Samia M. Ltaief; Wared Nour-Eldine; Janarthanan Ponraj; John Agcaoili; Said Mansour; Abeer R. Al-Shammari

doi:10.1016/j.micron.2023.103517

An optimized protocol for the preparation of blood immune cells for transmission electron microscopy

Nimshitha Pavathuparambil Abdul Manaph, Samia M. Ltaief, Wared Nour-Eldine, Janarthanan Ponraj, John Agcaoili, Said Mansour, Abeer R. Al-Shammari^*

^*Corresponding author for this work

Hamad bin Khalifa University

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Transmission electron microscopy (TEM) is a powerful technique that enables visualization of structural details inside cells. Prior to TEM imaging, biological samples must undergo several preparation steps that are optimized according to the sample type. Currently, there are limited protocols for the preparation of blood samples for TEM imaging. Here, we provide a detailed step-by-step method for preparing blood samples for TEM imaging. This protocol enables robust visualization of the ultrastructures of blood immune cells. In addition, we describe the typical cellular features that can be used to distinguish between different immune cells in the blood, such as neutrophils, eosinophils, monocytes, and lymphocytes. This protocol is useful for studying ultrastructural changes in blood immune cells under various physiological and disease conditions.

Original language	English
Article number	103517
Journal	Micron
Volume	173
DOIs	https://doi.org/10.1016/j.micron.2023.103517
Publication status	Published - Oct 2023

Keywords

Blood
Immune cells
Sample preparation
TEM
Transmission electron microscopy

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10.1016/j.micron.2023.103517

Cite this

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title = "An optimized protocol for the preparation of blood immune cells for transmission electron microscopy",

abstract = "Transmission electron microscopy (TEM) is a powerful technique that enables visualization of structural details inside cells. Prior to TEM imaging, biological samples must undergo several preparation steps that are optimized according to the sample type. Currently, there are limited protocols for the preparation of blood samples for TEM imaging. Here, we provide a detailed step-by-step method for preparing blood samples for TEM imaging. This protocol enables robust visualization of the ultrastructures of blood immune cells. In addition, we describe the typical cellular features that can be used to distinguish between different immune cells in the blood, such as neutrophils, eosinophils, monocytes, and lymphocytes. This protocol is useful for studying ultrastructural changes in blood immune cells under various physiological and disease conditions.",

keywords = "Blood, Immune cells, Sample preparation, TEM, Transmission electron microscopy",

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AU - Pavathuparambil Abdul Manaph, Nimshitha

AU - Ltaief, Samia M.

AU - Nour-Eldine, Wared

AU - Ponraj, Janarthanan

AU - Agcaoili, John

AU - Mansour, Said

AU - Al-Shammari, Abeer R.

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N2 - Transmission electron microscopy (TEM) is a powerful technique that enables visualization of structural details inside cells. Prior to TEM imaging, biological samples must undergo several preparation steps that are optimized according to the sample type. Currently, there are limited protocols for the preparation of blood samples for TEM imaging. Here, we provide a detailed step-by-step method for preparing blood samples for TEM imaging. This protocol enables robust visualization of the ultrastructures of blood immune cells. In addition, we describe the typical cellular features that can be used to distinguish between different immune cells in the blood, such as neutrophils, eosinophils, monocytes, and lymphocytes. This protocol is useful for studying ultrastructural changes in blood immune cells under various physiological and disease conditions.

AB - Transmission electron microscopy (TEM) is a powerful technique that enables visualization of structural details inside cells. Prior to TEM imaging, biological samples must undergo several preparation steps that are optimized according to the sample type. Currently, there are limited protocols for the preparation of blood samples for TEM imaging. Here, we provide a detailed step-by-step method for preparing blood samples for TEM imaging. This protocol enables robust visualization of the ultrastructures of blood immune cells. In addition, we describe the typical cellular features that can be used to distinguish between different immune cells in the blood, such as neutrophils, eosinophils, monocytes, and lymphocytes. This protocol is useful for studying ultrastructural changes in blood immune cells under various physiological and disease conditions.

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KW - Immune cells

KW - Sample preparation

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KW - Transmission electron microscopy

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An optimized protocol for the preparation of blood immune cells for transmission electron microscopy

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Keywords

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An optimized protocol for the preparation of blood immune cells for transmission electron microscopy

Abstract

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EX-QNRF-ECRA-2: Neuro-immune interactions in autism spectrum disorder

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