c-fos and ornithine decarboxylase gene expression in brain as early markers of neurotoxicity

Montserrat Vendrell; Nasser H. Zawia; Joan Serratosa; Stephen C. Bondy

doi:10.1016/0006-8993(91)90067-6

c-fos and ornithine decarboxylase gene expression in brain as early markers of neurotoxicity

Montserrat Vendrell^*, Nasser H. Zawia, Joan Serratosa, Stephen C. Bondy

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

An increase of proto-oncogene c-fos expression in cerebral cortex of rats treated with subconvulsant doses of the pesticide organochlorine lindane (γ-hexachlorocyclohexane) has been detected using Northern blots. Immunohistochemical studies show that Fos protein was already increased in neuronal nuclei 3 h after treatment. The administration of the benzodiazepine diazepam prior to lindane totally blocked the activation of this proto-oncogene expression. Parallel to this increased expression of c-fos an activation of the ornithine decarboxylase (ODC) gene and enzyme was also observed. High levels of ODC mRNA and increased enzyme activity in cortex were found in rats following lindane treatment. These changes were attenuated by prior treatment of animals with diazepam. The co-induction of c-fos and ODC suggests a potential link between the ODC/polyamine system and the short-acting proto-oncogenes in stimulus-transciption coupling events.

Original language	English
Pages (from-to)	291-296
Number of pages	6
Journal	Brain Research
Volume	544
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(91)90067-6
Publication status	Published - 29 Mar 1991
Externally published	Yes

Keywords

Cortex
Lindane
Neurotoxic
Ornithine decarboxylase
c-fos

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10.1016/0006-8993(91)90067-6

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title = "c-fos and ornithine decarboxylase gene expression in brain as early markers of neurotoxicity",

abstract = "An increase of proto-oncogene c-fos expression in cerebral cortex of rats treated with subconvulsant doses of the pesticide organochlorine lindane (γ-hexachlorocyclohexane) has been detected using Northern blots. Immunohistochemical studies show that Fos protein was already increased in neuronal nuclei 3 h after treatment. The administration of the benzodiazepine diazepam prior to lindane totally blocked the activation of this proto-oncogene expression. Parallel to this increased expression of c-fos an activation of the ornithine decarboxylase (ODC) gene and enzyme was also observed. High levels of ODC mRNA and increased enzyme activity in cortex were found in rats following lindane treatment. These changes were attenuated by prior treatment of animals with diazepam. The co-induction of c-fos and ODC suggests a potential link between the ODC/polyamine system and the short-acting proto-oncogenes in stimulus-transciption coupling events.",

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author = "Montserrat Vendrell and Zawia, {Nasser H.} and Joan Serratosa and Bondy, {Stephen C.}",

year = "1991",

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doi = "10.1016/0006-8993(91)90067-6",

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AU - Vendrell, Montserrat

AU - Zawia, Nasser H.

AU - Serratosa, Joan

AU - Bondy, Stephen C.

PY - 1991/3/29

Y1 - 1991/3/29

N2 - An increase of proto-oncogene c-fos expression in cerebral cortex of rats treated with subconvulsant doses of the pesticide organochlorine lindane (γ-hexachlorocyclohexane) has been detected using Northern blots. Immunohistochemical studies show that Fos protein was already increased in neuronal nuclei 3 h after treatment. The administration of the benzodiazepine diazepam prior to lindane totally blocked the activation of this proto-oncogene expression. Parallel to this increased expression of c-fos an activation of the ornithine decarboxylase (ODC) gene and enzyme was also observed. High levels of ODC mRNA and increased enzyme activity in cortex were found in rats following lindane treatment. These changes were attenuated by prior treatment of animals with diazepam. The co-induction of c-fos and ODC suggests a potential link between the ODC/polyamine system and the short-acting proto-oncogenes in stimulus-transciption coupling events.

AB - An increase of proto-oncogene c-fos expression in cerebral cortex of rats treated with subconvulsant doses of the pesticide organochlorine lindane (γ-hexachlorocyclohexane) has been detected using Northern blots. Immunohistochemical studies show that Fos protein was already increased in neuronal nuclei 3 h after treatment. The administration of the benzodiazepine diazepam prior to lindane totally blocked the activation of this proto-oncogene expression. Parallel to this increased expression of c-fos an activation of the ornithine decarboxylase (ODC) gene and enzyme was also observed. High levels of ODC mRNA and increased enzyme activity in cortex were found in rats following lindane treatment. These changes were attenuated by prior treatment of animals with diazepam. The co-induction of c-fos and ODC suggests a potential link between the ODC/polyamine system and the short-acting proto-oncogenes in stimulus-transciption coupling events.

KW - Cortex

KW - Lindane

KW - Neurotoxic

KW - Ornithine decarboxylase

KW - c-fos

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JF - Brain Research

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ER -

c-fos and ornithine decarboxylase gene expression in brain as early markers of neurotoxicity

Abstract

Keywords

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