TY - JOUR
T1 - Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a
AU - Teruel, Raúl
AU - Martínez-Martínez, Irene
AU - Guerrero, José A.
AU - González-Conejero, Rocío
AU - De La Morena-Barrio, María E.
AU - Salloum-Asfar, Salam
AU - Arroyo, Ana B.
AU - Águila, Sonia
AU - García-Barberá, Nuria
AU - Miñano, Antonia
AU - Vicente, Vicente
AU - Corral, Javier
AU - Martínez, Constantino
PY - 2013
Y1 - 2013
N2 - Background: Developmental haemostatic studies may help identifying new elements involved in the control of key haemostatic proteins like antithrombin, the most relevant endogenous anticoagulant. Results: In this study, we showed a significant reduction of sialic acid content in neonatal antithrombin compared with adult antithrombin in mice. mRNA levels of St3gal3 and St3gal4, two sialyltransferases potentially involved in antithrombin sialylation, were 85% lower in neonates in comparison with adults. In silico analysis of miRNAs overexpressed in neonates revealed that mir-200a might target these sialyltransferases. Moreover, in vitro studies in murine primary hepatocytes sustain this potential control. Conclusions: These data suggest that in addition to the direct protein regulation, microRNAs may also modulate qualitative traits of selected proteins by an indirect control of post-translational processes.
AB - Background: Developmental haemostatic studies may help identifying new elements involved in the control of key haemostatic proteins like antithrombin, the most relevant endogenous anticoagulant. Results: In this study, we showed a significant reduction of sialic acid content in neonatal antithrombin compared with adult antithrombin in mice. mRNA levels of St3gal3 and St3gal4, two sialyltransferases potentially involved in antithrombin sialylation, were 85% lower in neonates in comparison with adults. In silico analysis of miRNAs overexpressed in neonates revealed that mir-200a might target these sialyltransferases. Moreover, in vitro studies in murine primary hepatocytes sustain this potential control. Conclusions: These data suggest that in addition to the direct protein regulation, microRNAs may also modulate qualitative traits of selected proteins by an indirect control of post-translational processes.
KW - Antithrombin
KW - Microarray
KW - Post-natal development
KW - Post-translational modifications
KW - Sialytransferases
KW - miRNAs
UR - http://www.scopus.com/inward/record.url?scp=84878809389&partnerID=8YFLogxK
U2 - 10.1186/1423-0127-20-29
DO - 10.1186/1423-0127-20-29
M3 - Article
C2 - 23678987
AN - SCOPUS:84878809389
SN - 1021-7770
VL - 20
JO - Journal of Biomedical Science
JF - Journal of Biomedical Science
IS - 1
M1 - 29
ER -