Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4

Jiansong Ju; Jianxun Qi; Shujing Xu; Kouhei Ohnishi; Michael J. Benedik; Yanfen Xue; Yanhe Ma

doi:10.1107/S174430910900013X

Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4

Jiansong Ju, Jianxun Qi, Shujing Xu, Kouhei Ohnishi, Michael J. Benedik, Yanfen Xue, Yanhe Ma^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Alanine racemase (DadX_OF4), a dimeric endogenous PLP-dependent alkaline enzyme from alkaliphilic Bacillus pseudofirmus OF4, was expressed in Escherichia coli and purified with a His₆ tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 291 K using a solution containing 1.4 M sodium/potassium phosphate pH 8.2. The protein crystallized in space group P2₁2₁2₁, with two protein molecules in the asymmetric unit.

Original language	English
Pages (from-to)	166-168
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	65
Issue number	2
DOIs	https://doi.org/10.1107/S174430910900013X
Publication status	Published - 2009
Externally published	Yes

Keywords

Alkaline alanine racemase
Bacillus pseudofirmus OF4

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10.1107/S174430910900013X

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title = "Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4",

abstract = "Alanine racemase (DadXOF4), a dimeric endogenous PLP-dependent alkaline enzyme from alkaliphilic Bacillus pseudofirmus OF4, was expressed in Escherichia coli and purified with a His6 tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 291 K using a solution containing 1.4 M sodium/potassium phosphate pH 8.2. The protein crystallized in space group P212121, with two protein molecules in the asymmetric unit.",

keywords = "Alkaline alanine racemase, Bacillus pseudofirmus OF4",

author = "Jiansong Ju and Jianxun Qi and Shujing Xu and Kouhei Ohnishi and Benedik, {Michael J.} and Yanfen Xue and Yanhe Ma",

year = "2009",

doi = "10.1107/S174430910900013X",

language = "English",

volume = "65",

pages = "166--168",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

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T1 - Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4

AU - Ju, Jiansong

AU - Qi, Jianxun

AU - Xu, Shujing

AU - Ohnishi, Kouhei

AU - Benedik, Michael J.

AU - Xue, Yanfen

AU - Ma, Yanhe

PY - 2009

Y1 - 2009

N2 - Alanine racemase (DadXOF4), a dimeric endogenous PLP-dependent alkaline enzyme from alkaliphilic Bacillus pseudofirmus OF4, was expressed in Escherichia coli and purified with a His6 tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 291 K using a solution containing 1.4 M sodium/potassium phosphate pH 8.2. The protein crystallized in space group P212121, with two protein molecules in the asymmetric unit.

AB - Alanine racemase (DadXOF4), a dimeric endogenous PLP-dependent alkaline enzyme from alkaliphilic Bacillus pseudofirmus OF4, was expressed in Escherichia coli and purified with a His6 tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 291 K using a solution containing 1.4 M sodium/potassium phosphate pH 8.2. The protein crystallized in space group P212121, with two protein molecules in the asymmetric unit.

KW - Alkaline alanine racemase

KW - Bacillus pseudofirmus OF4

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U2 - 10.1107/S174430910900013X

DO - 10.1107/S174430910900013X

M3 - Article

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AN - SCOPUS:59649130463

SN - 1744-3091

VL - 65

SP - 166

EP - 168

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 2

ER -

Crystallization and preliminary X-ray study of alkaline alanine racemase from Bacillus pseudofirmus OF4

Abstract

Keywords

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