TY - JOUR
T1 - Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice
AU - Di Paolo, Gilbert
AU - Sankaranarayanan, Sethuraman
AU - Wenk, Markus R.
AU - Daniell, Laurie
AU - Perucco, Ezio
AU - Caldarone, Barbara J.
AU - Flavell, Richard
AU - Picciotto, Marina R.
AU - Ryan, Timothy A.
AU - Cremona, Ottavio
AU - De Camilli, Pietro
PY - 2002/2/28
Y1 - 2002/2/28
N2 - The function of the clathrin coat in synaptic vesicle endocytosis is assisted by a variety of accessory factors, among which amphiphysin (amphiphysin 1 and 2) is one of the best characterized. A putative endocytic function of amphiphysin was supported by dominant-negative interference studies. We have now generated amphiphysin 1 knockout mice and found that lack of amphiphysin 1 causes a parallel dramatic reduction of amphiphysin 2 selectively in brain. Cell-free assembly of endocytic protein scaffolds is defective in mutant brain extracts. Knockout mice exhibit defects in synaptic vesicle recycling that are unmasked by stimulation and suggest impairments at multiple stages of the cycle. These defects correlate with increased mortality due to rare irreversible seizures and with major learning deficits, suggesting a critical role of amphiphysin for higher brain functions.
AB - The function of the clathrin coat in synaptic vesicle endocytosis is assisted by a variety of accessory factors, among which amphiphysin (amphiphysin 1 and 2) is one of the best characterized. A putative endocytic function of amphiphysin was supported by dominant-negative interference studies. We have now generated amphiphysin 1 knockout mice and found that lack of amphiphysin 1 causes a parallel dramatic reduction of amphiphysin 2 selectively in brain. Cell-free assembly of endocytic protein scaffolds is defective in mutant brain extracts. Knockout mice exhibit defects in synaptic vesicle recycling that are unmasked by stimulation and suggest impairments at multiple stages of the cycle. These defects correlate with increased mortality due to rare irreversible seizures and with major learning deficits, suggesting a critical role of amphiphysin for higher brain functions.
UR - http://www.scopus.com/inward/record.url?scp=18344376169&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(02)00601-3
DO - 10.1016/S0896-6273(02)00601-3
M3 - Article
C2 - 11879655
AN - SCOPUS:18344376169
SN - 0896-6273
VL - 33
SP - 789
EP - 804
JO - Neuron
JF - Neuron
IS - 5
ER -