Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence

Chris Wai Tung Leung; Feng Guo; Yuning Hong; Engui Zhao; Ryan Tsz Kin Kwok; Nelson Lik Ching Leung; Sijie Chen; Nishant N. Vaikath; Omar Mukhtar El-Agnaf; Youhong Tang; Wei Ping Gai; Ben Zhong Tang

doi:10.1039/c4cc07911f

Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence

Chris Wai Tung Leung, Feng Guo, Yuning Hong, Engui Zhao, Ryan Tsz Kin Kwok, Nelson Lik Ching Leung, Sijie Chen, Nishant N. Vaikath, Omar Mukhtar El-Agnaf, Youhong Tang^*, Wei Ping Gai, Ben Zhong Tang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

We report a fluorophore, TPE-TPP, with AIE characteristics which is utilized as a fluorescence probe to monitor the α-synuclein (α-Syn) fibrillation process. Compared with ThT, TPE-TPP shows a higher sensitivity in the detection of α-Syn oligomers as well as fibrils with a stronger fluorescence. The performance of TPE-TPP was evaluated using fluorescence, AFM, dot blot, and SEC.

Original language	English
Pages (from-to)	1866-1869
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	10
DOIs	https://doi.org/10.1039/c4cc07911f
Publication status	Published - 4 Feb 2015
Externally published	Yes

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title = "Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence",

abstract = "We report a fluorophore, TPE-TPP, with AIE characteristics which is utilized as a fluorescence probe to monitor the α-synuclein (α-Syn) fibrillation process. Compared with ThT, TPE-TPP shows a higher sensitivity in the detection of α-Syn oligomers as well as fibrils with a stronger fluorescence. The performance of TPE-TPP was evaluated using fluorescence, AFM, dot blot, and SEC.",

author = "Leung, {Chris Wai Tung} and Feng Guo and Yuning Hong and Engui Zhao and Kwok, {Ryan Tsz Kin} and Leung, {Nelson Lik Ching} and Sijie Chen and Vaikath, {Nishant N.} and El-Agnaf, {Omar Mukhtar} and Youhong Tang and Gai, {Wei Ping} and Tang, {Ben Zhong}",

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doi = "10.1039/c4cc07911f",

language = "English",

volume = "51",

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T1 - Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence

AU - Leung, Chris Wai Tung

AU - Guo, Feng

AU - Hong, Yuning

AU - Zhao, Engui

AU - Kwok, Ryan Tsz Kin

AU - Leung, Nelson Lik Ching

AU - Chen, Sijie

AU - Vaikath, Nishant N.

AU - El-Agnaf, Omar Mukhtar

AU - Tang, Youhong

AU - Gai, Wei Ping

AU - Tang, Ben Zhong

PY - 2015/2/4

Y1 - 2015/2/4

N2 - We report a fluorophore, TPE-TPP, with AIE characteristics which is utilized as a fluorescence probe to monitor the α-synuclein (α-Syn) fibrillation process. Compared with ThT, TPE-TPP shows a higher sensitivity in the detection of α-Syn oligomers as well as fibrils with a stronger fluorescence. The performance of TPE-TPP was evaluated using fluorescence, AFM, dot blot, and SEC.

AB - We report a fluorophore, TPE-TPP, with AIE characteristics which is utilized as a fluorescence probe to monitor the α-synuclein (α-Syn) fibrillation process. Compared with ThT, TPE-TPP shows a higher sensitivity in the detection of α-Syn oligomers as well as fibrils with a stronger fluorescence. The performance of TPE-TPP was evaluated using fluorescence, AFM, dot blot, and SEC.

UR - http://www.scopus.com/inward/record.url?scp=84921506541&partnerID=8YFLogxK

U2 - 10.1039/c4cc07911f

DO - 10.1039/c4cc07911f

M3 - Article

C2 - 25526628

AN - SCOPUS:84921506541

SN - 1359-7345

VL - 51

SP - 1866

EP - 1869

JO - Chemical Communications

JF - Chemical Communications

IS - 10

ER -

Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence

Abstract

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