Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells

Gowher Ali; Essam M. Abdelalim

doi:10.1016/j.xpro.2022.101613

Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells

Gowher Ali^*, Essam M. Abdelalim^*

^*Corresponding author for this work

QBRI - Diabetes Research Center

Hamad bin Khalifa University

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The human pluripotent stem cell (hPSC) differentiation has allowed for the generation of in vitro models to study human diseases in a dish. This protocol describes the generation of keratinocyte-like cells from hPSCs in chemically defined media. Treating hPSCs with retinoic acid and BMP4 induced the generation of keratinocyte progenitors, which further differentiated into mature keratinocytes in the presence of calcium. The keratinocytes generated with this protocol could be used to study keratinocyte biology, drug screening, and skin-related diseases. For complete details on the use and execution of this protocol, please refer to Ali et al. (2020).

Original language	English
Article number	101613
Journal	STAR Protocols
Volume	3
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2022.101613
Publication status	Published - 16 Sept 2022

Keywords

Cell culture
Cell differentiation
Stem cells

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10.1016/j.xpro.2022.101613

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abstract = "The human pluripotent stem cell (hPSC) differentiation has allowed for the generation of in vitro models to study human diseases in a dish. This protocol describes the generation of keratinocyte-like cells from hPSCs in chemically defined media. Treating hPSCs with retinoic acid and BMP4 induced the generation of keratinocyte progenitors, which further differentiated into mature keratinocytes in the presence of calcium. The keratinocytes generated with this protocol could be used to study keratinocyte biology, drug screening, and skin-related diseases. For complete details on the use and execution of this protocol, please refer to Ali et al. (2020).",

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AB - The human pluripotent stem cell (hPSC) differentiation has allowed for the generation of in vitro models to study human diseases in a dish. This protocol describes the generation of keratinocyte-like cells from hPSCs in chemically defined media. Treating hPSCs with retinoic acid and BMP4 induced the generation of keratinocyte progenitors, which further differentiated into mature keratinocytes in the presence of calcium. The keratinocytes generated with this protocol could be used to study keratinocyte biology, drug screening, and skin-related diseases. For complete details on the use and execution of this protocol, please refer to Ali et al. (2020).

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KW - Cell differentiation

KW - Stem cells

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Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells

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Keywords

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