Distribution of Secretory Phospholipase A2 XIIA in the Brain and its Role in Lipid Metabolism and Cognition

Sze Min Ee; Yew Long Lo; Guanghou Shui; Markus R. Wenk; Eun Joo Shin; Hyoung Chun Kim; Wei Yi Ong

doi:10.1007/s12035-014-8635-7

Distribution of Secretory Phospholipase A2 XIIA in the Brain and its Role in Lipid Metabolism and Cognition

Sze Min Ee, Yew Long Lo, Guanghou Shui, Markus R. Wenk, Eun Joo Shin, Hyoung Chun Kim, Wei Yi Ong^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Phospholipases A₂(PLA₂) catalyze the hydrolysis of membrane phospholipids to produce free fatty acids and lysophospholipids, which have important functions in cell signaling. The present study elucidated differential expression of PLA₂isoforms in the rat cortex by quantitative reverse transcription PCR and demonstrated that sPLA₂-XIIA had greater messenger RNA expression than iPLA₂-VI or cPLA₂-IVA in different brain regions, or compared to other sPLA₂isoforms in the prefrontal cortex (PFC) and hippocampus. Western blots identified a 24-kDa band in different regions of the adult brain, and high levels of sPLA₂-XIIA protein expression were detected in the PFC, striatum, and thalamus. Electron microscopy showed that sPLA₂-XIIA is present in axon terminals and dendrites. Injection of antisense oligonucleotide to sPLA₂-XIIA in the PFC and lipidomic analysis showed increase in phospholipid but decrease in lysophospholipid species consistent with decreased catalytic activity of the enzyme, changes in arachidonic acid release, and alterations in sphingolipids. sPLA₂-XIIA knockdown also resulted in shorter latency timings in the passive avoidance test, and higher number of errors in the attention set-shifting task, indicating deficits in working memory and attention. Together, the results show an important role of sPLA₂-XIIA in lipid metabolism, prefrontal cortical function, and cognition.

Original language	English
Pages (from-to)	60-75
Number of pages	16
Journal	Molecular Neurobiology
Volume	50
Issue number	1
DOIs	https://doi.org/10.1007/s12035-014-8635-7
Publication status	Published - 2 Oct 2014
Externally published	Yes

Keywords

Attention
Cognition
Electron microscopy
Lipidomics
Secretory phospholipase A2

Access to Document

10.1007/s12035-014-8635-7

Cite this

@article{2470fd86f880422eace736880518ee2f,

title = "Distribution of Secretory Phospholipase A2 XIIA in the Brain and its Role in Lipid Metabolism and Cognition",

abstract = "Phospholipases A2(PLA2) catalyze the hydrolysis of membrane phospholipids to produce free fatty acids and lysophospholipids, which have important functions in cell signaling. The present study elucidated differential expression of PLA2isoforms in the rat cortex by quantitative reverse transcription PCR and demonstrated that sPLA2-XIIA had greater messenger RNA expression than iPLA2-VI or cPLA2-IVA in different brain regions, or compared to other sPLA2isoforms in the prefrontal cortex (PFC) and hippocampus. Western blots identified a 24-kDa band in different regions of the adult brain, and high levels of sPLA2-XIIA protein expression were detected in the PFC, striatum, and thalamus. Electron microscopy showed that sPLA2-XIIA is present in axon terminals and dendrites. Injection of antisense oligonucleotide to sPLA2-XIIA in the PFC and lipidomic analysis showed increase in phospholipid but decrease in lysophospholipid species consistent with decreased catalytic activity of the enzyme, changes in arachidonic acid release, and alterations in sphingolipids. sPLA2-XIIA knockdown also resulted in shorter latency timings in the passive avoidance test, and higher number of errors in the attention set-shifting task, indicating deficits in working memory and attention. Together, the results show an important role of sPLA2-XIIA in lipid metabolism, prefrontal cortical function, and cognition.",

keywords = "Attention, Cognition, Electron microscopy, Lipidomics, Secretory phospholipase A2",

author = "Ee, {Sze Min} and Lo, {Yew Long} and Guanghou Shui and Wenk, {Markus R.} and Shin, {Eun Joo} and Kim, {Hyoung Chun} and Ong, {Wei Yi}",

note = "Publisher Copyright: {\textcopyright} 2014, Springer Science+Business Media New York.",

year = "2014",

month = oct,

day = "2",

doi = "10.1007/s12035-014-8635-7",

language = "English",

volume = "50",

pages = "60--75",

journal = "Molecular Neurobiology",

issn = "0893-7648",

publisher = "Springer",

number = "1",

}

TY - JOUR

T1 - Distribution of Secretory Phospholipase A2 XIIA in the Brain and its Role in Lipid Metabolism and Cognition

AU - Ee, Sze Min

AU - Lo, Yew Long

AU - Shui, Guanghou

AU - Wenk, Markus R.

AU - Shin, Eun Joo

AU - Kim, Hyoung Chun

AU - Ong, Wei Yi

PY - 2014/10/2

Y1 - 2014/10/2

N2 - Phospholipases A2(PLA2) catalyze the hydrolysis of membrane phospholipids to produce free fatty acids and lysophospholipids, which have important functions in cell signaling. The present study elucidated differential expression of PLA2isoforms in the rat cortex by quantitative reverse transcription PCR and demonstrated that sPLA2-XIIA had greater messenger RNA expression than iPLA2-VI or cPLA2-IVA in different brain regions, or compared to other sPLA2isoforms in the prefrontal cortex (PFC) and hippocampus. Western blots identified a 24-kDa band in different regions of the adult brain, and high levels of sPLA2-XIIA protein expression were detected in the PFC, striatum, and thalamus. Electron microscopy showed that sPLA2-XIIA is present in axon terminals and dendrites. Injection of antisense oligonucleotide to sPLA2-XIIA in the PFC and lipidomic analysis showed increase in phospholipid but decrease in lysophospholipid species consistent with decreased catalytic activity of the enzyme, changes in arachidonic acid release, and alterations in sphingolipids. sPLA2-XIIA knockdown also resulted in shorter latency timings in the passive avoidance test, and higher number of errors in the attention set-shifting task, indicating deficits in working memory and attention. Together, the results show an important role of sPLA2-XIIA in lipid metabolism, prefrontal cortical function, and cognition.

AB - Phospholipases A2(PLA2) catalyze the hydrolysis of membrane phospholipids to produce free fatty acids and lysophospholipids, which have important functions in cell signaling. The present study elucidated differential expression of PLA2isoforms in the rat cortex by quantitative reverse transcription PCR and demonstrated that sPLA2-XIIA had greater messenger RNA expression than iPLA2-VI or cPLA2-IVA in different brain regions, or compared to other sPLA2isoforms in the prefrontal cortex (PFC) and hippocampus. Western blots identified a 24-kDa band in different regions of the adult brain, and high levels of sPLA2-XIIA protein expression were detected in the PFC, striatum, and thalamus. Electron microscopy showed that sPLA2-XIIA is present in axon terminals and dendrites. Injection of antisense oligonucleotide to sPLA2-XIIA in the PFC and lipidomic analysis showed increase in phospholipid but decrease in lysophospholipid species consistent with decreased catalytic activity of the enzyme, changes in arachidonic acid release, and alterations in sphingolipids. sPLA2-XIIA knockdown also resulted in shorter latency timings in the passive avoidance test, and higher number of errors in the attention set-shifting task, indicating deficits in working memory and attention. Together, the results show an important role of sPLA2-XIIA in lipid metabolism, prefrontal cortical function, and cognition.

KW - Attention

KW - Cognition

KW - Electron microscopy

KW - Lipidomics

KW - Secretory phospholipase A2

UR - http://www.scopus.com/inward/record.url?scp=84911002270&partnerID=8YFLogxK

U2 - 10.1007/s12035-014-8635-7

DO - 10.1007/s12035-014-8635-7

M3 - Article

C2 - 24464264

AN - SCOPUS:84911002270

SN - 0893-7648

VL - 50

SP - 60

EP - 75

JO - Molecular Neurobiology

JF - Molecular Neurobiology

IS - 1

ER -

Distribution of Secretory Phospholipase A2 XIIA in the Brain and its Role in Lipid Metabolism and Cognition

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this