TY - JOUR
T1 - Effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein E e{open}3 and e{open}4 knock-in mice
AU - Lim, Wei Ling Florence
AU - Lam, Sin Man
AU - Shui, Guanghou
AU - Mondal, Alinda
AU - Ong, Daniel
AU - Duan, Xinrui
AU - Creegan, Rhona
AU - Martins, Ian J.
AU - Sharman, Matthew J.
AU - Taddei, Kevin
AU - Verdile, Giuseppe
AU - Wenk, Markus R.
AU - Martins, Ralph Nigel
PY - 2013/9
Y1 - 2013/9
N2 - Apolipoprotein E (ApoE) is important in facilitating the transport of lipids (cholesterol, phospholipids, and sulfatides) and plays a fundamental role in normal lipid metabolism. High cholesterol levels increases the risk of developing Alzheimer's disease. In this study, we investigated the effects of a high-fat high cholesterol (HFHC) diet on brain lipid profiles in 95 young and aged APOE e{open}3 and e{open}4 knock-in mice to determine whether diet leads to altered brain levels of a number of glycerophospholipids, sphingolipids, cholesterol precursors, cholesterol, cholesterol oxidation products, and cholesterol esters. The results in this study revealed significant changes in lipid levels. The HFHC-enriched diet influenced the levels of cholesterol esters. A sharp increase in cholesterol ester levels, particularly in the aged APOE e{open}4 diet-enriched group, might be suggestive of abnormal acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT) activity and/or levels. Age exerts appreciable effects on the brain lipidome, especially with regard to polar lipid species.
AB - Apolipoprotein E (ApoE) is important in facilitating the transport of lipids (cholesterol, phospholipids, and sulfatides) and plays a fundamental role in normal lipid metabolism. High cholesterol levels increases the risk of developing Alzheimer's disease. In this study, we investigated the effects of a high-fat high cholesterol (HFHC) diet on brain lipid profiles in 95 young and aged APOE e{open}3 and e{open}4 knock-in mice to determine whether diet leads to altered brain levels of a number of glycerophospholipids, sphingolipids, cholesterol precursors, cholesterol, cholesterol oxidation products, and cholesterol esters. The results in this study revealed significant changes in lipid levels. The HFHC-enriched diet influenced the levels of cholesterol esters. A sharp increase in cholesterol ester levels, particularly in the aged APOE e{open}4 diet-enriched group, might be suggestive of abnormal acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT) activity and/or levels. Age exerts appreciable effects on the brain lipidome, especially with regard to polar lipid species.
KW - APOE KI mice
KW - Glycerophospholipids
KW - High cholesterol
KW - High fat
KW - Mass spectrometry
KW - Oxysterols
UR - http://www.scopus.com/inward/record.url?scp=84878900198&partnerID=8YFLogxK
U2 - 10.1016/j.neurobiolaging.2013.03.012
DO - 10.1016/j.neurobiolaging.2013.03.012
M3 - Article
C2 - 23601809
AN - SCOPUS:84878900198
SN - 0197-4580
VL - 34
SP - 2217
EP - 2224
JO - Neurobiology of Aging
JF - Neurobiology of Aging
IS - 9
ER -