Exploring the in vivo anti-cancer potential of Neosetophomone B in leukemic cells using a zebrafish xenograft model

Shilpa Kuttikrishnan; Maram Hasan; Kirti S. Prabhu; Tamam El-Elimat; Nicholas H. Oberlies; Cedric J. Pearce; Feras Q. Alali; Aamir Ahmad; Ehsan Pourkarimi; Ajaz A. Bhat; Huseyin C. Yalcin; Shahab Uddin

doi:10.1016/j.yexcr.2023.113907

Exploring the in vivo anti-cancer potential of Neosetophomone B in leukemic cells using a zebrafish xenograft model

Shilpa Kuttikrishnan, Maram Hasan, Kirti S. Prabhu, Tamam El-Elimat, Nicholas H. Oberlies, Cedric J. Pearce, Feras Q. Alali, Aamir Ahmad, Ehsan Pourkarimi, Ajaz A. Bhat, Huseyin C. Yalcin^*, Shahab Uddin^*

^*Corresponding author for this work

Genomics and Translational Biomedicine

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Neosetophomone B (NSP–B) is a unique meroterpenoid fungal secondary metabolite that has previously demonstrated promising anti-cancer properties against various cancer cell lines in vitro. However, its in vivo anti-cancer potential remaines unexplored. To fill this gap in our knowledge, we tested NSP-B's in vivo anti-cancer activity using a zebrafish model, an organism that has gained significant traction in biomedical research due to its genetic similarities with humans and its transparent nature, allowing real-time tumor growth observation. For our experiments, we employed the K562-injected zebrafish xenograft model. Upon treating these zebrafish with NSP-B, we observed a marked reduction in the size and number of tumor xenografts. Delving deeper, our analyses indicated that NSP-B curtailed tumor growth and proliferation of leukemic grafted xenograft within the zebrafish. These results show that NSP-B possesses potent in vivo anti-cancer properties, making it a potential novel therapeutic agent for addressing hematological malignancies.

Original language	English
Article number	113907
Number of pages	5
Journal	Experimental Cell Research
Volume	435
Issue number	1
DOIs	https://doi.org/10.1016/j.yexcr.2023.113907
Publication status	Published - 1 Feb 2024

Keywords

Cell proliferation
Fungal secondary metabolites
Leukemia
Neosetophomone B
Xenograft model
Zebrafish

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Kuttikrishnan, S., Hasan, M., Prabhu, K. S., El-Elimat, T., Oberlies, N. H., Pearce, C. J., Alali, F. Q., Ahmad, A., Pourkarimi, E., Bhat, A. A., Yalcin, H. C., & Uddin, S. (2024). Exploring the in vivo anti-cancer potential of Neosetophomone B in leukemic cells using a zebrafish xenograft model. Experimental Cell Research, 435(1), Article 113907. https://doi.org/10.1016/j.yexcr.2023.113907

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title = "Exploring the in vivo anti-cancer potential of Neosetophomone B in leukemic cells using a zebrafish xenograft model",

abstract = "Neosetophomone B (NSP–B) is a unique meroterpenoid fungal secondary metabolite that has previously demonstrated promising anti-cancer properties against various cancer cell lines in vitro. However, its in vivo anti-cancer potential remaines unexplored. To fill this gap in our knowledge, we tested NSP-B's in vivo anti-cancer activity using a zebrafish model, an organism that has gained significant traction in biomedical research due to its genetic similarities with humans and its transparent nature, allowing real-time tumor growth observation. For our experiments, we employed the K562-injected zebrafish xenograft model. Upon treating these zebrafish with NSP-B, we observed a marked reduction in the size and number of tumor xenografts. Delving deeper, our analyses indicated that NSP-B curtailed tumor growth and proliferation of leukemic grafted xenograft within the zebrafish. These results show that NSP-B possesses potent in vivo anti-cancer properties, making it a potential novel therapeutic agent for addressing hematological malignancies.",

keywords = "Cell proliferation, Fungal secondary metabolites, Leukemia, Neosetophomone B, Xenograft model, Zebrafish",

author = "Shilpa Kuttikrishnan and Maram Hasan and Prabhu, {Kirti S.} and Tamam El-Elimat and Oberlies, {Nicholas H.} and Pearce, {Cedric J.} and Alali, {Feras Q.} and Aamir Ahmad and Ehsan Pourkarimi and Bhat, {Ajaz A.} and Yalcin, {Huseyin C.} and Shahab Uddin",

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AU - El-Elimat, Tamam

AU - Oberlies, Nicholas H.

AU - Pearce, Cedric J.

AU - Alali, Feras Q.

AU - Ahmad, Aamir

AU - Pourkarimi, Ehsan

AU - Bhat, Ajaz A.

AU - Yalcin, Huseyin C.

AU - Uddin, Shahab

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N2 - Neosetophomone B (NSP–B) is a unique meroterpenoid fungal secondary metabolite that has previously demonstrated promising anti-cancer properties against various cancer cell lines in vitro. However, its in vivo anti-cancer potential remaines unexplored. To fill this gap in our knowledge, we tested NSP-B's in vivo anti-cancer activity using a zebrafish model, an organism that has gained significant traction in biomedical research due to its genetic similarities with humans and its transparent nature, allowing real-time tumor growth observation. For our experiments, we employed the K562-injected zebrafish xenograft model. Upon treating these zebrafish with NSP-B, we observed a marked reduction in the size and number of tumor xenografts. Delving deeper, our analyses indicated that NSP-B curtailed tumor growth and proliferation of leukemic grafted xenograft within the zebrafish. These results show that NSP-B possesses potent in vivo anti-cancer properties, making it a potential novel therapeutic agent for addressing hematological malignancies.

AB - Neosetophomone B (NSP–B) is a unique meroterpenoid fungal secondary metabolite that has previously demonstrated promising anti-cancer properties against various cancer cell lines in vitro. However, its in vivo anti-cancer potential remaines unexplored. To fill this gap in our knowledge, we tested NSP-B's in vivo anti-cancer activity using a zebrafish model, an organism that has gained significant traction in biomedical research due to its genetic similarities with humans and its transparent nature, allowing real-time tumor growth observation. For our experiments, we employed the K562-injected zebrafish xenograft model. Upon treating these zebrafish with NSP-B, we observed a marked reduction in the size and number of tumor xenografts. Delving deeper, our analyses indicated that NSP-B curtailed tumor growth and proliferation of leukemic grafted xenograft within the zebrafish. These results show that NSP-B possesses potent in vivo anti-cancer properties, making it a potential novel therapeutic agent for addressing hematological malignancies.

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Exploring the in vivo anti-cancer potential of Neosetophomone B in leukemic cells using a zebrafish xenograft model

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