Fabrication of a glycation induced amyloid nanofibril and polyalizarin yellow R nanobiocomposite: Application for electrocatalytic determination of hydrogen peroxide

Nader Amini*, Mohammad Bagher Gholivand, Mojtaba Shamsipur, Ali Akbar Moosavi Movahedi, Sara Farahi, Mehran Habibi-Rezaei, Afshin Maleki, Reza Rezaee, Kazhal Naderi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Amyloid fibrils were produced in a solution of bovine serum albumin (BSA) in buffer solution in the presence of fructose. The solution was incubated for 20 weeks in the dark. We used glycation induced bovine serum albumin in which fibrilogenesis (nano fibrils) followed by using fluorescence (Thioflavin T), Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM) to achieve the size and morphology of fibrils. A novel electrochemical biosensor for the detection of hydrogen peroxide was developed based on immobilizing poly (alizarin yellow R) and amyloid nano-fibrils on glassy carbon electrode (PAYR/AMLNFibs/GCE). The electrocatalytic response of the biosensor was proportional to the hydrogen peroxide concentration in the range of 1 μM to 2.2 mM with a limit of detection and sensitivity of 290 nM and 0.024 μA/μM, respectively. The modified electrode demonstrated many advantages such as high sensitivity, low detection of limit and excellent catalytic activity.

Original languageEnglish
Pages (from-to)1297-1304
Number of pages8
JournalInternational Journal of Biological Macromolecules
Volume123
DOIs
Publication statusPublished - 15 Feb 2019
Externally publishedYes

Keywords

  • Alizarin yellow R
  • Amyloid nanofibrils
  • Electrochemical biosensor
  • Glycation
  • Hydrogen peroxide

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