TY - JOUR
T1 - Fabrication of a glycation induced amyloid nanofibril and polyalizarin yellow R nanobiocomposite
T2 - Application for electrocatalytic determination of hydrogen peroxide
AU - Amini, Nader
AU - Gholivand, Mohammad Bagher
AU - Shamsipur, Mojtaba
AU - Movahedi, Ali Akbar Moosavi
AU - Farahi, Sara
AU - Habibi-Rezaei, Mehran
AU - Maleki, Afshin
AU - Rezaee, Reza
AU - Naderi, Kazhal
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/2/15
Y1 - 2019/2/15
N2 - Amyloid fibrils were produced in a solution of bovine serum albumin (BSA) in buffer solution in the presence of fructose. The solution was incubated for 20 weeks in the dark. We used glycation induced bovine serum albumin in which fibrilogenesis (nano fibrils) followed by using fluorescence (Thioflavin T), Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM) to achieve the size and morphology of fibrils. A novel electrochemical biosensor for the detection of hydrogen peroxide was developed based on immobilizing poly (alizarin yellow R) and amyloid nano-fibrils on glassy carbon electrode (PAYR/AMLNFibs/GCE). The electrocatalytic response of the biosensor was proportional to the hydrogen peroxide concentration in the range of 1 μM to 2.2 mM with a limit of detection and sensitivity of 290 nM and 0.024 μA/μM, respectively. The modified electrode demonstrated many advantages such as high sensitivity, low detection of limit and excellent catalytic activity.
AB - Amyloid fibrils were produced in a solution of bovine serum albumin (BSA) in buffer solution in the presence of fructose. The solution was incubated for 20 weeks in the dark. We used glycation induced bovine serum albumin in which fibrilogenesis (nano fibrils) followed by using fluorescence (Thioflavin T), Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM) to achieve the size and morphology of fibrils. A novel electrochemical biosensor for the detection of hydrogen peroxide was developed based on immobilizing poly (alizarin yellow R) and amyloid nano-fibrils on glassy carbon electrode (PAYR/AMLNFibs/GCE). The electrocatalytic response of the biosensor was proportional to the hydrogen peroxide concentration in the range of 1 μM to 2.2 mM with a limit of detection and sensitivity of 290 nM and 0.024 μA/μM, respectively. The modified electrode demonstrated many advantages such as high sensitivity, low detection of limit and excellent catalytic activity.
KW - Alizarin yellow R
KW - Amyloid nanofibrils
KW - Electrochemical biosensor
KW - Glycation
KW - Hydrogen peroxide
UR - http://www.scopus.com/inward/record.url?scp=85055558897&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2018.10.043
DO - 10.1016/j.ijbiomac.2018.10.043
M3 - Article
C2 - 30336241
AN - SCOPUS:85055558897
SN - 0141-8130
VL - 123
SP - 1297
EP - 1304
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -