TY - JOUR
T1 - FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC
AU - Charles Richard, John Lalith
AU - Shukla, Manu Shubhdarshan
AU - Menoni, Hervé
AU - Ouararhni, Khalid
AU - Lone, Imtiaz Nisar
AU - Roulland, Yohan
AU - Papin, Christophe
AU - Ben Simon, Elsa
AU - Kundu, Tapas
AU - Hamiche, Ali
AU - Angelov, Dimitar
AU - Dimitrov, Stefan
N1 - Publisher Copyright:
© 2016 Charles Richard et al.
PY - 2016/7
Y1 - 2016/7
N2 - FACT, in addition to its role in transcription, is likely implicated in both transcription-coupled nucleotide excision repair and DNA double strand break repair. Here, we present evidence that FACT could be directly involved in Base Excision Repair and elucidate the chromatin remodeling mechanisms of FACT during BER. We found that, upon oxidative stress, FACT is released from transcription related protein complexes to get associated with repair proteins and chromatin remodelers from the SWI/SNF family. We also showed the rapid recruitment of FACT to the site of damage, coincident with the glycosylase OGG1, upon the local generation of oxidized DNA. Interestingly, FACT facilitates uracil-DNA glycosylase in the removal of uracil from nucleosomal DNA thanks to an enhancement in the remodeling activity of RSC. This discloses a novel property of FACT wherein it has a co-remodeling activity and strongly enhances the remodeling capacity of the chromatin remodelers. Altogether, our data suggest that FACT may acts in concert with RSC to facilitate excision of DNA lesions during the initial step of BER.
AB - FACT, in addition to its role in transcription, is likely implicated in both transcription-coupled nucleotide excision repair and DNA double strand break repair. Here, we present evidence that FACT could be directly involved in Base Excision Repair and elucidate the chromatin remodeling mechanisms of FACT during BER. We found that, upon oxidative stress, FACT is released from transcription related protein complexes to get associated with repair proteins and chromatin remodelers from the SWI/SNF family. We also showed the rapid recruitment of FACT to the site of damage, coincident with the glycosylase OGG1, upon the local generation of oxidized DNA. Interestingly, FACT facilitates uracil-DNA glycosylase in the removal of uracil from nucleosomal DNA thanks to an enhancement in the remodeling activity of RSC. This discloses a novel property of FACT wherein it has a co-remodeling activity and strongly enhances the remodeling capacity of the chromatin remodelers. Altogether, our data suggest that FACT may acts in concert with RSC to facilitate excision of DNA lesions during the initial step of BER.
UR - http://www.scopus.com/inward/record.url?scp=84982782574&partnerID=8YFLogxK
U2 - 10.1371/journal.pgen.1006221
DO - 10.1371/journal.pgen.1006221
M3 - Article
C2 - 27467129
AN - SCOPUS:84982782574
SN - 1553-7390
VL - 12
JO - PLoS Genetics
JF - PLoS Genetics
IS - 7
M1 - e1006221
ER -