Generation of sibling-matched induced pluripotent stem cell lines from spinal and bulbar muscular atrophy patients

Gunaseelan Narayanan; Marianne Sheila; Josiah Chai; Lawrence W. Stanton

doi:10.1016/j.scr.2017.02.005

Generation of sibling-matched induced pluripotent stem cell lines from spinal and bulbar muscular atrophy patients

Gunaseelan Narayanan, Marianne Sheila, Josiah Chai, Lawrence W. Stanton^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Spinal and bulbar muscular atrophy (SBMA) is a neurodegenerative disease caused by the expansion of CAG repeats in the Androgen Receptor gene (AR). We report the generation of induced pluripotent stem cell (iPSC) lines from two SBMA patients and their healthy siblings. The SBMA and healthy iPSC lines retain the number of AR CAG repeats, express pluripotency markers and are able to differentiate into the three germ layers. The iPSC lines are also free of Sendai virus transgenes and have normal karyotypes. The SBMA iPSC lines with their sibling-matched controls would serve as useful tools to study SBMA disease mechanism.

Original language	English
Pages (from-to)	30-33
Number of pages	4
Journal	Stem Cell Research
Volume	20
DOIs	https://doi.org/10.1016/j.scr.2017.02.005
Publication status	Published - 1 Apr 2017
Externally published	Yes

Keywords

Androgen receptor
Induced pluripotent stem cells
Spinal and bulbar muscular atrophy

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10.1016/j.scr.2017.02.005

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title = "Generation of sibling-matched induced pluripotent stem cell lines from spinal and bulbar muscular atrophy patients",

abstract = "Spinal and bulbar muscular atrophy (SBMA) is a neurodegenerative disease caused by the expansion of CAG repeats in the Androgen Receptor gene (AR). We report the generation of induced pluripotent stem cell (iPSC) lines from two SBMA patients and their healthy siblings. The SBMA and healthy iPSC lines retain the number of AR CAG repeats, express pluripotency markers and are able to differentiate into the three germ layers. The iPSC lines are also free of Sendai virus transgenes and have normal karyotypes. The SBMA iPSC lines with their sibling-matched controls would serve as useful tools to study SBMA disease mechanism.",

keywords = "Androgen receptor, Induced pluripotent stem cells, Spinal and bulbar muscular atrophy",

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AU - Narayanan, Gunaseelan

AU - Sheila, Marianne

AU - Chai, Josiah

AU - Stanton, Lawrence W.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Spinal and bulbar muscular atrophy (SBMA) is a neurodegenerative disease caused by the expansion of CAG repeats in the Androgen Receptor gene (AR). We report the generation of induced pluripotent stem cell (iPSC) lines from two SBMA patients and their healthy siblings. The SBMA and healthy iPSC lines retain the number of AR CAG repeats, express pluripotency markers and are able to differentiate into the three germ layers. The iPSC lines are also free of Sendai virus transgenes and have normal karyotypes. The SBMA iPSC lines with their sibling-matched controls would serve as useful tools to study SBMA disease mechanism.

AB - Spinal and bulbar muscular atrophy (SBMA) is a neurodegenerative disease caused by the expansion of CAG repeats in the Androgen Receptor gene (AR). We report the generation of induced pluripotent stem cell (iPSC) lines from two SBMA patients and their healthy siblings. The SBMA and healthy iPSC lines retain the number of AR CAG repeats, express pluripotency markers and are able to differentiate into the three germ layers. The iPSC lines are also free of Sendai virus transgenes and have normal karyotypes. The SBMA iPSC lines with their sibling-matched controls would serve as useful tools to study SBMA disease mechanism.

KW - Androgen receptor

KW - Induced pluripotent stem cells

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SN - 1873-5061

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SP - 30

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JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of sibling-matched induced pluripotent stem cell lines from spinal and bulbar muscular atrophy patients

Abstract

Keywords

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