GNAO1 Mutations Affecting the N-Terminal α-Helix of Gαo Lead to Parkinsonism

Background: Patients carrying pathogenic variants in GNAO1 present a phenotypic spectrum ranging from severe early-onset epileptic encephalopathy and developmental delay to mild adolescent/adult-onset dystonia. Genotype-phenotype correlation and molecular mechanisms underlying the disease remain understudied.Methods: We analyzed the clinical course of a child carrying the novel GNAO1 mutation c.38T>C;p.Leu13Pro, and structural, biochemical, and cellular properties of the corresponding mutant G alpha o-GNAO1-encoded protein-alongside the related mutation c.68T>C;p.Leu23Pro.Results: The main clinical feature was parkinsonism with bradykinesia and rigidity, unlike the hyperkinetic movement disorder commonly associated with GNAO1 mutations. The Leu -> Pro substitutions have no impact on enzymatic activity or overall folding of G alpha o but uniquely destabilize the N-terminal alpha-helix, blocking formation of the heterotrimeric G-protein and disabling activation by G-protein-coupled receptors.Conclusions: Our study defines a parkinsonism phenotype within the spectrum of GNAO1 disorders and suggests a genotype-phenotype correlation by GNAO1 mutations targeting the N-terminal alpha-helix of G alpha o. (c) 2024 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.