Imaging the Pharynx to Measure the Uptake of Doxorubicin in Caenorhabditis elegans

Sivathevy Amirthagunabalasingam; Arturo Papaluca; Taramatti Harihar; Dindial Ramotar

doi:10.21769/BioProtoc.2291

Imaging the Pharynx to Measure the Uptake of Doxorubicin in Caenorhabditis elegans

Sivathevy Amirthagunabalasingam, Arturo Papaluca, Taramatti Harihar, Dindial Ramotar

Research output: Contribution to journal › Article › peer-review

Abstract

Caenorhabditis elegans offers an array of advantages to investigate the roles of uptake transporters. Herein, an epifluorescent microscopy approach was developed to monitor the uptake of the autofluorescent anticancer drug, doxorubicin, into the pharynx of C. elegans by organic cation transporters.

Original language	English
Article number	e2291
Number of pages	14
Journal	Bio-protocol
Volume	7
Issue number	10
DOIs	https://doi.org/10.21769/BioProtoc.2291
Publication status	Published - 20 May 2017
Externally published	Yes

Keywords

Autofluorescent drug
C. elegans
Doxorubicin
Drug uptake
Epifluorescent microscopy
Organic cation uptake transporters
RNAi feeding bacteria

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10.21769/BioProtoc.2291

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Imaging the Pharynx to Measure the Uptake of Doxorubicin in Caenorhabditis elegans

Abstract

Keywords

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