TY - JOUR
T1 - In Vitro expression of natriuretic peptides in cardiomyocytes differentiated from monkey embryonic stem cells
AU - Abdelalim, Essam Mohamed
AU - Takada, Tatsuyuki
AU - Toyoda, Futoshi
AU - Omatsu-Kanbe, Mariko
AU - Matsuura, Hiroshi
AU - Tooyama, Ikuo
AU - Torii, Ryuzo
PY - 2006/2/10
Y1 - 2006/2/10
N2 - Functional characterization of ES cell-derived cardiomyocytes is important for differentiation control and application to the cell therapy. One of the crucial functions of cardiomyocytes is a production of atrial and brain natriuretic peptides (ANP and BNP, respectively), which have important endocrine, autocrine, and paracrine functions. In this study, we focused on the functional aspect of the cardiomyocytes differentiated from monkey ES cells in vitro and investigated the expression of ANP and BNP. Spontaneously contracting cells showed nodal-like action potentials, and expression of ANP and BNP by RT-PCR and immunocytochemistry. Interestingly, ANP and BNP expressions were detected as immunoreactive granules in the perinuclear area and these signals appeared to co-localize with trans-Golgi network. These findings suggest that monkey ES cells were able to differentiate into cardiomyocytes with functional characteristics in vitro and therefore can be used as a useful model to study mechanisms and functions in early cardiogenesis.
AB - Functional characterization of ES cell-derived cardiomyocytes is important for differentiation control and application to the cell therapy. One of the crucial functions of cardiomyocytes is a production of atrial and brain natriuretic peptides (ANP and BNP, respectively), which have important endocrine, autocrine, and paracrine functions. In this study, we focused on the functional aspect of the cardiomyocytes differentiated from monkey ES cells in vitro and investigated the expression of ANP and BNP. Spontaneously contracting cells showed nodal-like action potentials, and expression of ANP and BNP by RT-PCR and immunocytochemistry. Interestingly, ANP and BNP expressions were detected as immunoreactive granules in the perinuclear area and these signals appeared to co-localize with trans-Golgi network. These findings suggest that monkey ES cells were able to differentiate into cardiomyocytes with functional characteristics in vitro and therefore can be used as a useful model to study mechanisms and functions in early cardiogenesis.
KW - ANP
KW - BNP
KW - Cardiomyocytes
KW - Expression
KW - In vitro
KW - Monkey ES cells
UR - http://www.scopus.com/inward/record.url?scp=29644435426&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.12.057
DO - 10.1016/j.bbrc.2005.12.057
M3 - Article
C2 - 16378593
AN - SCOPUS:29644435426
SN - 0006-291X
VL - 340
SP - 689
EP - 695
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -