Integrated study of globally expressed microRNAs in IL-1β-stimulated human osteoarthritis chondrocytes and osteoarthritis relevant genes: A microarray and bioinformatics analysis

Zafar Rasheed; Hani A. Al-Shobaili; Naila Rasheed; Abdulaziz A.M. Al Salloom; Osama Al-Shaya; Amer Mahmood; Nehad M. Alajez; Ahmed S.S. Alghamdi; El Sayed E. Mehana

doi:10.1080/15257770.2016.1163380

Integrated study of globally expressed microRNAs in IL-1β-stimulated human osteoarthritis chondrocytes and osteoarthritis relevant genes: A microarray and bioinformatics analysis

Zafar Rasheed^*, Hani A. Al-Shobaili, Naila Rasheed, Abdulaziz A.M. Al Salloom, Osama Al-Shaya, Amer Mahmood, Nehad M. Alajez, Ahmed S.S. Alghamdi, El Sayed E. Mehana

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

This study was undertaken to identify and characterize the globally expressed microRNAs (miRNAs) involved in interleukin-1β (IL-1β)-induced joint damage and to predict whether miRNAs can regulate the catabolic effects in osteoarthritis (OA) chondrocytes. Out of 1347 miRNAs analyzed by microarrays in IL-1β-stimulated OA chondrocytes, 35 miRNAs were down-regulated, 1 miRNA was up-regulated, and the expression of 1311 miRNAs remained unchanged. Bioinformatics analysis showed the key inflammatory mediators and key molecular pathways are targeted by differentially expressed miRNAs. Novel miRNAs identified could have important diagnostic and therapeutic potentials in the development of novel therapeutic strategies for pain managements in OA.

Original language	English
Pages (from-to)	335-355
Number of pages	21
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	35
Issue number	7
DOIs	https://doi.org/10.1080/15257770.2016.1163380
Publication status	Published - 2 Jul 2016
Externally published	Yes

Keywords

Bioinformatics
Chondrocytes
Microarray
Osteoarthritis
microRNAs

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10.1080/15257770.2016.1163380

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Rasheed, Z., Al-Shobaili, H. A., Rasheed, N., Al Salloom, A. A. M., Al-Shaya, O., Mahmood, A., Alajez, N. M., Alghamdi, A. S. S., & Mehana, E. S. E. (2016). Integrated study of globally expressed microRNAs in IL-1β-stimulated human osteoarthritis chondrocytes and osteoarthritis relevant genes: A microarray and bioinformatics analysis. Nucleosides, Nucleotides and Nucleic Acids, 35(7), 335-355. https://doi.org/10.1080/15257770.2016.1163380

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title = "Integrated study of globally expressed microRNAs in IL-1β-stimulated human osteoarthritis chondrocytes and osteoarthritis relevant genes: A microarray and bioinformatics analysis",

abstract = "This study was undertaken to identify and characterize the globally expressed microRNAs (miRNAs) involved in interleukin-1β (IL-1β)-induced joint damage and to predict whether miRNAs can regulate the catabolic effects in osteoarthritis (OA) chondrocytes. Out of 1347 miRNAs analyzed by microarrays in IL-1β-stimulated OA chondrocytes, 35 miRNAs were down-regulated, 1 miRNA was up-regulated, and the expression of 1311 miRNAs remained unchanged. Bioinformatics analysis showed the key inflammatory mediators and key molecular pathways are targeted by differentially expressed miRNAs. Novel miRNAs identified could have important diagnostic and therapeutic potentials in the development of novel therapeutic strategies for pain managements in OA.",

keywords = "Bioinformatics, Chondrocytes, Microarray, Osteoarthritis, microRNAs",

author = "Zafar Rasheed and Al-Shobaili, {Hani A.} and Naila Rasheed and {Al Salloom}, {Abdulaziz A.M.} and Osama Al-Shaya and Amer Mahmood and Alajez, {Nehad M.} and Alghamdi, {Ahmed S.S.} and Mehana, {El Sayed E.}",

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doi = "10.1080/15257770.2016.1163380",

language = "English",

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Rasheed, Z, Al-Shobaili, HA, Rasheed, N, Al Salloom, AAM, Al-Shaya, O, Mahmood, A, Alajez, NM, Alghamdi, ASS & Mehana, ESE 2016, 'Integrated study of globally expressed microRNAs in IL-1β-stimulated human osteoarthritis chondrocytes and osteoarthritis relevant genes: A microarray and bioinformatics analysis', Nucleosides, Nucleotides and Nucleic Acids, vol. 35, no. 7, pp. 335-355. https://doi.org/10.1080/15257770.2016.1163380

Integrated study of globally expressed microRNAs in IL-1β-stimulated human osteoarthritis chondrocytes and osteoarthritis relevant genes: A microarray and bioinformatics analysis. / Rasheed, Zafar; Al-Shobaili, Hani A.; Rasheed, Naila et al.
In: Nucleosides, Nucleotides and Nucleic Acids, Vol. 35, No. 7, 02.07.2016, p. 335-355.

Research output: Contribution to journal › Article › peer-review

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T1 - Integrated study of globally expressed microRNAs in IL-1β-stimulated human osteoarthritis chondrocytes and osteoarthritis relevant genes

T2 - A microarray and bioinformatics analysis

AU - Rasheed, Zafar

AU - Al-Shobaili, Hani A.

AU - Rasheed, Naila

AU - Al Salloom, Abdulaziz A.M.

AU - Al-Shaya, Osama

AU - Mahmood, Amer

AU - Alajez, Nehad M.

AU - Alghamdi, Ahmed S.S.

AU - Mehana, El Sayed E.

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AB - This study was undertaken to identify and characterize the globally expressed microRNAs (miRNAs) involved in interleukin-1β (IL-1β)-induced joint damage and to predict whether miRNAs can regulate the catabolic effects in osteoarthritis (OA) chondrocytes. Out of 1347 miRNAs analyzed by microarrays in IL-1β-stimulated OA chondrocytes, 35 miRNAs were down-regulated, 1 miRNA was up-regulated, and the expression of 1311 miRNAs remained unchanged. Bioinformatics analysis showed the key inflammatory mediators and key molecular pathways are targeted by differentially expressed miRNAs. Novel miRNAs identified could have important diagnostic and therapeutic potentials in the development of novel therapeutic strategies for pain managements in OA.

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KW - Chondrocytes

KW - Microarray

KW - Osteoarthritis

KW - microRNAs

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Integrated study of globally expressed microRNAs in IL-1β-stimulated human osteoarthritis chondrocytes and osteoarthritis relevant genes: A microarray and bioinformatics analysis

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