KDM6B histone demethylase is an epigenetic regulator of estrogen receptor β expression in human pleural mesothelioma

Arcangela G. Manente; Giulia Pinton; Sara Zonca; Daniela Tavian; Tanwir Habib; Puthen V. Jithesh; Dean Fennell; Stefan Nilsson; Laura Moro

doi:10.2217/epi-2016-0025

KDM6B histone demethylase is an epigenetic regulator of estrogen receptor β expression in human pleural mesothelioma

Arcangela G. Manente, Giulia Pinton, Sara Zonca, Daniela Tavian, Tanwir Habib, Puthen V. Jithesh, Dean Fennell, Stefan Nilsson, Laura Moro^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Aim: To assess the correlation between KDM6B and estrogen receptor β (ERβ) expression in malignant pleural mesothelioma (MPM). Materials & methods: We evaluated gene expression by in silico analysis of microarray data, real-time PCR and western blot in MPM tumors and cell lines. Results & conclusion: We report a strong positive correlation between the expression of KDM6B and ERβ in MPM tumors and cell lines. We describe that, in hypoxia, the HIF2α-KDM6B axis induces an epithelioid morphology and ERβ expression in biphasic MPM cells with estrogen receptor-negative phenotype. Reduced histone H3K27 tri-methylation confirms KDM6B activity under hypoxic conditions. Importantly, cells treated during reoxygenation with the selective ERβ agonist, KB9520, maintain ERβ expression and the less aggressive phenotype acquired in hypoxia.

Original language	English
Pages (from-to)	1227-1238
Number of pages	12
Journal	Epigenomics
Volume	8
Issue number	9
DOIs	https://doi.org/10.2217/epi-2016-0025
Publication status	Published - Sept 2016
Externally published	Yes

Keywords

ERβ
H3K27me3
KDM6B
epigenetic
human malignant pleural mesothelioma

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title = "KDM6B histone demethylase is an epigenetic regulator of estrogen receptor β expression in human pleural mesothelioma",

abstract = "Aim: To assess the correlation between KDM6B and estrogen receptor β (ERβ) expression in malignant pleural mesothelioma (MPM). Materials & methods: We evaluated gene expression by in silico analysis of microarray data, real-time PCR and western blot in MPM tumors and cell lines. Results & conclusion: We report a strong positive correlation between the expression of KDM6B and ERβ in MPM tumors and cell lines. We describe that, in hypoxia, the HIF2α-KDM6B axis induces an epithelioid morphology and ERβ expression in biphasic MPM cells with estrogen receptor-negative phenotype. Reduced histone H3K27 tri-methylation confirms KDM6B activity under hypoxic conditions. Importantly, cells treated during reoxygenation with the selective ERβ agonist, KB9520, maintain ERβ expression and the less aggressive phenotype acquired in hypoxia.",

keywords = "ERβ, H3K27me3, KDM6B, epigenetic, human malignant pleural mesothelioma",

author = "Manente, {Arcangela G.} and Giulia Pinton and Sara Zonca and Daniela Tavian and Tanwir Habib and Jithesh, {Puthen V.} and Dean Fennell and Stefan Nilsson and Laura Moro",

note = "Publisher Copyright: {\textcopyright} 2016 Laura Moro.",

year = "2016",

month = sep,

doi = "10.2217/epi-2016-0025",

language = "English",

volume = "8",

pages = "1227--1238",

journal = "Epigenomics",

issn = "1750-1911",

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T1 - KDM6B histone demethylase is an epigenetic regulator of estrogen receptor β expression in human pleural mesothelioma

AU - Manente, Arcangela G.

AU - Pinton, Giulia

AU - Zonca, Sara

AU - Tavian, Daniela

AU - Habib, Tanwir

AU - Jithesh, Puthen V.

AU - Fennell, Dean

AU - Nilsson, Stefan

AU - Moro, Laura

PY - 2016/9

Y1 - 2016/9

N2 - Aim: To assess the correlation between KDM6B and estrogen receptor β (ERβ) expression in malignant pleural mesothelioma (MPM). Materials & methods: We evaluated gene expression by in silico analysis of microarray data, real-time PCR and western blot in MPM tumors and cell lines. Results & conclusion: We report a strong positive correlation between the expression of KDM6B and ERβ in MPM tumors and cell lines. We describe that, in hypoxia, the HIF2α-KDM6B axis induces an epithelioid morphology and ERβ expression in biphasic MPM cells with estrogen receptor-negative phenotype. Reduced histone H3K27 tri-methylation confirms KDM6B activity under hypoxic conditions. Importantly, cells treated during reoxygenation with the selective ERβ agonist, KB9520, maintain ERβ expression and the less aggressive phenotype acquired in hypoxia.

AB - Aim: To assess the correlation between KDM6B and estrogen receptor β (ERβ) expression in malignant pleural mesothelioma (MPM). Materials & methods: We evaluated gene expression by in silico analysis of microarray data, real-time PCR and western blot in MPM tumors and cell lines. Results & conclusion: We report a strong positive correlation between the expression of KDM6B and ERβ in MPM tumors and cell lines. We describe that, in hypoxia, the HIF2α-KDM6B axis induces an epithelioid morphology and ERβ expression in biphasic MPM cells with estrogen receptor-negative phenotype. Reduced histone H3K27 tri-methylation confirms KDM6B activity under hypoxic conditions. Importantly, cells treated during reoxygenation with the selective ERβ agonist, KB9520, maintain ERβ expression and the less aggressive phenotype acquired in hypoxia.

KW - ERβ

KW - H3K27me3

KW - KDM6B

KW - epigenetic

KW - human malignant pleural mesothelioma

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U2 - 10.2217/epi-2016-0025

DO - 10.2217/epi-2016-0025

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AN - SCOPUS:84986907132

SN - 1750-1911

VL - 8

SP - 1227

EP - 1238

JO - Epigenomics

JF - Epigenomics

IS - 9

ER -

KDM6B histone demethylase is an epigenetic regulator of estrogen receptor β expression in human pleural mesothelioma

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Keywords

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