TY - JOUR
T1 - Lipid profiling of C. elegans strains administered pro-longevity drugs and drug combinations
AU - Admasu, Tesfahun Dessale
AU - Batchu, Krishna Chaithanya
AU - Ng, Li Fang
AU - Cazenave-Gassiot, Amaury
AU - Wenk, Markus R.
AU - Gruber, Jan
N1 - Publisher Copyright:
© The Author(s) 2018.
PY - 2018
Y1 - 2018
N2 - We report the effect of four lifespan modifying drugs and of synergistic combinations of these drugs on lipid profile in Caenorhabditis elegans. We employ ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) to compare the abundance of lipid species in treated and control animals. Adult nematodes were treated with rapamycin, rifampicin, psora-4 and allantoin and combinations of these compounds and the resulting change in lipid profiles, specifically in those of triacylglycerol (TAG), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were determined. We quantified changes resulting from treatment with the drug combinations relative to untreated controls and relative to animals treated with each constituent single drugs. We further determined the dependence of changes in lipid profiles on genes known to affect lipid metabolism using strains carrying mutations in these pathways. In particular, we determined lipid profiles in a genetic model of caloric restriction (eat-2), a strain lacking homolog of TGFβ (daf-7) and in a strain lacking the SREBP/sbp-1 transcription factor.
AB - We report the effect of four lifespan modifying drugs and of synergistic combinations of these drugs on lipid profile in Caenorhabditis elegans. We employ ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) to compare the abundance of lipid species in treated and control animals. Adult nematodes were treated with rapamycin, rifampicin, psora-4 and allantoin and combinations of these compounds and the resulting change in lipid profiles, specifically in those of triacylglycerol (TAG), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were determined. We quantified changes resulting from treatment with the drug combinations relative to untreated controls and relative to animals treated with each constituent single drugs. We further determined the dependence of changes in lipid profiles on genes known to affect lipid metabolism using strains carrying mutations in these pathways. In particular, we determined lipid profiles in a genetic model of caloric restriction (eat-2), a strain lacking homolog of TGFβ (daf-7) and in a strain lacking the SREBP/sbp-1 transcription factor.
UR - http://www.scopus.com/inward/record.url?scp=85055181902&partnerID=8YFLogxK
U2 - 10.1038/sdata.2018.231
DO - 10.1038/sdata.2018.231
M3 - Article
C2 - 30351306
AN - SCOPUS:85055181902
SN - 2052-4463
VL - 5
JO - Scientific data
JF - Scientific data
M1 - 180231
ER -