TY - JOUR
T1 - Lipidomic "deep profiling"
T2 - An enhanced workflow to reveal new molecular species of signaling lipids
AU - Narayanaswamy, Pradeep
AU - Shinde, Sudhirkumar
AU - Sulc, Robert
AU - Kraut, Rachel
AU - Staples, Gregory
AU - Thiam, Chung Hwee
AU - Grimm, Rudolf
AU - Sellergren, Börje
AU - Torta, Federico
AU - Wenk, Markus R.
PY - 2014/3/18
Y1 - 2014/3/18
N2 - Current mass spectrometry-based lipidomics aims to comprehensively cover wide ranges of lipid classes. We introduce a strategy to capture phospho-monoester lipids and improve the detection of long-chain base phosphates (LCB-Ps, e.g., sphingosine-1-phosphate). Ten novel LCB-Ps (d18:2, t20:1, odd carbon forms) were discovered and characterized in tissues from human and mouse, as well in D. melanogaster and S. cerevisiae. These findings have immediate relevance for our understanding of sphingosine-1-phosphate biosynthesis, signaling, and degradation.
AB - Current mass spectrometry-based lipidomics aims to comprehensively cover wide ranges of lipid classes. We introduce a strategy to capture phospho-monoester lipids and improve the detection of long-chain base phosphates (LCB-Ps, e.g., sphingosine-1-phosphate). Ten novel LCB-Ps (d18:2, t20:1, odd carbon forms) were discovered and characterized in tissues from human and mouse, as well in D. melanogaster and S. cerevisiae. These findings have immediate relevance for our understanding of sphingosine-1-phosphate biosynthesis, signaling, and degradation.
UR - http://www.scopus.com/inward/record.url?scp=84896511361&partnerID=8YFLogxK
U2 - 10.1021/ac4039652
DO - 10.1021/ac4039652
M3 - Article
C2 - 24533588
AN - SCOPUS:84896511361
SN - 0003-2700
VL - 86
SP - 3043
EP - 3047
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 6
ER -