Lipidomic "deep profiling": An enhanced workflow to reveal new molecular species of signaling lipids

Pradeep Narayanaswamy, Sudhirkumar Shinde, Robert Sulc, Rachel Kraut, Gregory Staples, Chung Hwee Thiam, Rudolf Grimm, Börje Sellergren, Federico Torta*, Markus R. Wenk

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

71 Citations (Scopus)

Abstract

Current mass spectrometry-based lipidomics aims to comprehensively cover wide ranges of lipid classes. We introduce a strategy to capture phospho-monoester lipids and improve the detection of long-chain base phosphates (LCB-Ps, e.g., sphingosine-1-phosphate). Ten novel LCB-Ps (d18:2, t20:1, odd carbon forms) were discovered and characterized in tissues from human and mouse, as well in D. melanogaster and S. cerevisiae. These findings have immediate relevance for our understanding of sphingosine-1-phosphate biosynthesis, signaling, and degradation.

Original languageEnglish
Pages (from-to)3043-3047
Number of pages5
JournalAnalytical Chemistry
Volume86
Issue number6
DOIs
Publication statusPublished - 18 Mar 2014
Externally publishedYes

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