TY - JOUR
T1 - Metabolic inflexibility in individuals with obesity assessed by near-infrared spectroscopy
AU - Soares, Rogério Nogueira
AU - Reimer, Raylene A.
AU - Doyle-Baker, Patricia K.
AU - Murias, Juan M.
N1 - Publisher Copyright:
© SAGE Publications.
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Objective: To non-invasively evaluate differences in oxidative metabolism in individuals with obesity compared to normal weight using the near-infrared spectroscopy and vascular occlusion technique during hyperglycaemia. Methods: In all, 16 normal-weight individuals (body mass index: 21.3 ± 1.7 kg/m2) and 13 individuals with obesity (body mass index: 34.4 ± 2.0 kg/m2) had five vascular occlusion tests (pre, 30, 60, 90 and 120 min after glucose ingestion). Oxygen utilization was estimated from the area under the curve of the deoxyhemoglobin [HHb] signal during occlusion. Muscle reperfusion was derived from the area above the curve after cuff release. Results: The deoxyhemoglobin area under the curve during occlusion of the normal-weight individuals increased from 15,732 ± 2344 (%. s) at pre to 18,930 ± 3226 (%. s) (p < 0.05) at 90 min after glucose ingestion. The deoxyhemoglobin area under the curve during occlusion decreased significantly from 14,695 ± 3341 (%. s) at pre to 11,273 ± 1825 (%. s) (p < 0.05) and 11,360 ± 1750 (%. s) (p < 0.05) at 30 and 60 min, respectively, after glucose ingestion. The area above the curve of deoxyhemoglobin during reperfusion decreased significantly from 6450 ± 765 (%. s) at pre to 4830 ± 963 (%. s) (p < 0.05) at 60 min and to 4210 ± 595 (%. s) (p < 0.01) at 90 min in normal-weight individuals after glucose ingestion, with no changes observed in individuals with obesity. Conclusion: This study confirmed in vivo and non-invasively the metabolic inflexibility of skeletal muscle in individuals with obesity during hyperglycaemia.
AB - Objective: To non-invasively evaluate differences in oxidative metabolism in individuals with obesity compared to normal weight using the near-infrared spectroscopy and vascular occlusion technique during hyperglycaemia. Methods: In all, 16 normal-weight individuals (body mass index: 21.3 ± 1.7 kg/m2) and 13 individuals with obesity (body mass index: 34.4 ± 2.0 kg/m2) had five vascular occlusion tests (pre, 30, 60, 90 and 120 min after glucose ingestion). Oxygen utilization was estimated from the area under the curve of the deoxyhemoglobin [HHb] signal during occlusion. Muscle reperfusion was derived from the area above the curve after cuff release. Results: The deoxyhemoglobin area under the curve during occlusion of the normal-weight individuals increased from 15,732 ± 2344 (%. s) at pre to 18,930 ± 3226 (%. s) (p < 0.05) at 90 min after glucose ingestion. The deoxyhemoglobin area under the curve during occlusion decreased significantly from 14,695 ± 3341 (%. s) at pre to 11,273 ± 1825 (%. s) (p < 0.05) and 11,360 ± 1750 (%. s) (p < 0.05) at 30 and 60 min, respectively, after glucose ingestion. The area above the curve of deoxyhemoglobin during reperfusion decreased significantly from 6450 ± 765 (%. s) at pre to 4830 ± 963 (%. s) (p < 0.05) at 60 min and to 4210 ± 595 (%. s) (p < 0.01) at 90 min in normal-weight individuals after glucose ingestion, with no changes observed in individuals with obesity. Conclusion: This study confirmed in vivo and non-invasively the metabolic inflexibility of skeletal muscle in individuals with obesity during hyperglycaemia.
KW - Glucose
KW - glucose metabolism
KW - glucose oxidation
KW - metabolic diseases
KW - obesity
UR - http://www.scopus.com/inward/record.url?scp=85032869537&partnerID=8YFLogxK
U2 - 10.1177/1479164117725478
DO - 10.1177/1479164117725478
M3 - Article
C2 - 28825331
AN - SCOPUS:85032869537
SN - 1479-1641
VL - 14
SP - 502
EP - 509
JO - Diabetes and Vascular Disease Research
JF - Diabetes and Vascular Disease Research
IS - 6
ER -