TY - JOUR
T1 - Methylomic profiling in trisomy 21 identifies cognition-and Alzheimer's disease-related dysregulation
AU - Haertle, Larissa
AU - Müller, Tobias
AU - Lardenoije, Roy
AU - Maierhofer, Anna
AU - Dittrich, Marcus
AU - Riemens, Renzo J.M.
AU - Stora, Samantha
AU - Roche, Mathilde
AU - Leber, Markus
AU - Riedel-Heller, Steffi
AU - Wagner, Michael
AU - Scherer, Martin
AU - Ravel, Aimé
AU - Mircher, Clotilde
AU - Cieuta-Walti, Cecile
AU - Durand, Sophie
AU - Van De Hove, Daniel L.A.
AU - Hoffmann, Per
AU - Ramirez, Alfredo
AU - Haaf, Thomas
AU - El Hajj, Nady
AU - Mégarbané, André
N1 - Publisher Copyright:
© 2019 The Author(s).
PY - 2019/12/16
Y1 - 2019/12/16
N2 - Background: Trisomy 21 (T21) is associated with intellectual disability that ranges from mild to profound with an average intellectual quotient of around 50. Furthermore, T21 patients have a high risk of developing Alzheimer's disease (AD) early in life, characterized by the presence of senile plaques of amyloid protein and neurofibrillary tangles, leading to neuronal loss and cognitive decline. We postulate that epigenetic factors contribute to the observed variability in intellectual disability, as well as at the level of neurodegeneration seen in T21 individuals. Materials and Methods: A genome-wide DNA methylation study was performed using Illumina Infinium® MethylationEPIC BeadChips on whole blood DNA of 3 male T21 patients with low IQ, 8 T21 patients with high IQ (4 males and 4 females), and 21 age-and sex-matched control samples (12 males and 9 females) in order to determine whether DNA methylation alterations could help explain variation in cognitive impairment between individuals with T21. In view of the increased risk of developing AD in T21 individuals, we additionally investigated the T21-associated sites in published blood DNA methylation data from the AgeCoDe cohort (German study on Ageing, Cognition, and Dementia). AgeCoDe represents a prospective longitudinal study including non-demented individuals at baseline of which a part develops AD dementia at follow-up. Results: Two thousand seven hundred sixteen differentially methylated sites and regions discriminating T21 and healthy individuals were identified. In the T21 high and low IQ comparison, a single CpG located in the promoter of PELI1 was differentially methylated after multiple testing adjustment. For the same contrast, 69 differentially methylated regions were identified. Performing a targeted association analysis for the significant T21-associated CpG sites in the AgeCoDe cohort, we found that 9 showed significant methylation differences related to AD dementia, including one in the ADAM10 gene. This gene has previously been shown to play a role in the prevention of amyloid plaque formation in the brain. Conclusion: The differentially methylated regions may help understand the interaction between methylation alterations and cognitive function. In addition, ADAM10 might be a valuable blood-based biomarker for at least the early detection of AD.
AB - Background: Trisomy 21 (T21) is associated with intellectual disability that ranges from mild to profound with an average intellectual quotient of around 50. Furthermore, T21 patients have a high risk of developing Alzheimer's disease (AD) early in life, characterized by the presence of senile plaques of amyloid protein and neurofibrillary tangles, leading to neuronal loss and cognitive decline. We postulate that epigenetic factors contribute to the observed variability in intellectual disability, as well as at the level of neurodegeneration seen in T21 individuals. Materials and Methods: A genome-wide DNA methylation study was performed using Illumina Infinium® MethylationEPIC BeadChips on whole blood DNA of 3 male T21 patients with low IQ, 8 T21 patients with high IQ (4 males and 4 females), and 21 age-and sex-matched control samples (12 males and 9 females) in order to determine whether DNA methylation alterations could help explain variation in cognitive impairment between individuals with T21. In view of the increased risk of developing AD in T21 individuals, we additionally investigated the T21-associated sites in published blood DNA methylation data from the AgeCoDe cohort (German study on Ageing, Cognition, and Dementia). AgeCoDe represents a prospective longitudinal study including non-demented individuals at baseline of which a part develops AD dementia at follow-up. Results: Two thousand seven hundred sixteen differentially methylated sites and regions discriminating T21 and healthy individuals were identified. In the T21 high and low IQ comparison, a single CpG located in the promoter of PELI1 was differentially methylated after multiple testing adjustment. For the same contrast, 69 differentially methylated regions were identified. Performing a targeted association analysis for the significant T21-associated CpG sites in the AgeCoDe cohort, we found that 9 showed significant methylation differences related to AD dementia, including one in the ADAM10 gene. This gene has previously been shown to play a role in the prevention of amyloid plaque formation in the brain. Conclusion: The differentially methylated regions may help understand the interaction between methylation alterations and cognitive function. In addition, ADAM10 might be a valuable blood-based biomarker for at least the early detection of AD.
KW - Alzheimer's disease
KW - Cognitive function
KW - DNA methylation
KW - Down syndrome
KW - Infinium Methylation EPIC arrays
KW - Intellectual disability
KW - Trisomy 21
UR - http://www.scopus.com/inward/record.url?scp=85076710593&partnerID=8YFLogxK
U2 - 10.1186/s13148-019-0787-x
DO - 10.1186/s13148-019-0787-x
M3 - Article
C2 - 31843015
AN - SCOPUS:85076710593
SN - 1868-7075
VL - 11
JO - Clinical Epigenetics
JF - Clinical Epigenetics
IS - 1
M1 - 195
ER -