TY - JOUR
T1 - Nucleotide sequence and properties of the murine γ3 immunoglobulin heavy chain gene switch region
T2 - Implications for successive Cγ gene switching
AU - Stanton, Lawrence W.
AU - Marcu, Kenneth B.
PY - 1982/10/11
Y1 - 1982/10/11
N2 - During B lymphocyte differentiation, inmmoglobulin heavy chain constant region (CH) genes undergo a unique series of DNA recombination events culminating in the CH class switch. CH switch (S) regions are located 2 kb 5′ of each CH gene except δ (i.e. μ, γ3, γl, γ2b, γ2a, e{open} and α). We describe the structural features of the γ3 switch region. Hybridization experiments show that Sγ3 has remarkable homology to both Sμ and other Sγ regions while Sμ possesses limited homology to the other Sγ sequences. However, Sμ possesses extensive sequence homology with Se{open} and Sα. The nucleotide sequence of Sγ3 reveals higher densities of Sμ repetitive sequences (GAGCT and GGGGT) and another S region cammon sequence (YAGGTTG) than observed for Sγ1 , Sγ2b or Sγ2a. In addition, the conservation of Sμ like repetitive sequences in Sγ regions is correlated with the 543′ → 3′ γ gene order (i.e. Sγ3>Sγ1>Sγ2b>Sγ2a). A model is presented which suggests that the unique features of Sγ3 may allow for successive switches from Cμ to any Cγ gene.
AB - During B lymphocyte differentiation, inmmoglobulin heavy chain constant region (CH) genes undergo a unique series of DNA recombination events culminating in the CH class switch. CH switch (S) regions are located 2 kb 5′ of each CH gene except δ (i.e. μ, γ3, γl, γ2b, γ2a, e{open} and α). We describe the structural features of the γ3 switch region. Hybridization experiments show that Sγ3 has remarkable homology to both Sμ and other Sγ regions while Sμ possesses limited homology to the other Sγ sequences. However, Sμ possesses extensive sequence homology with Se{open} and Sα. The nucleotide sequence of Sγ3 reveals higher densities of Sμ repetitive sequences (GAGCT and GGGGT) and another S region cammon sequence (YAGGTTG) than observed for Sγ1 , Sγ2b or Sγ2a. In addition, the conservation of Sμ like repetitive sequences in Sγ regions is correlated with the 543′ → 3′ γ gene order (i.e. Sγ3>Sγ1>Sγ2b>Sγ2a). A model is presented which suggests that the unique features of Sγ3 may allow for successive switches from Cμ to any Cγ gene.
UR - http://www.scopus.com/inward/record.url?scp=0020480604&partnerID=8YFLogxK
U2 - 10.1093/nar/10.19.5993
DO - 10.1093/nar/10.19.5993
M3 - Article
C2 - 6292864
AN - SCOPUS:0020480604
SN - 0305-1048
VL - 10
SP - 5993
EP - 6006
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 19
ER -