Abstract
Films of surface-bound homo-oligonucleotides on gold were hybridized and subjected to rinses using various volumes of water in preparation for ex situ spectroscopic analysis performed by high-resolution X-ray photoelectron spectroscopy, near-edge X-ray absorption fine structure spectroscopy, and infrared reflection-absorption spectroscopy. It was found that films rinsed with 0.5 mL of water contained well-ordered DNA hybrids, while films rinsed with greater volumes (1 and 2 mL) showed a decreased number of hybrids and a significant disruption of their intactness and order. These results illustrate the importance of taking into account preparation procedures when interpreting ex situ spectroscopic data of such systems.
Original language | English |
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Pages (from-to) | 267-270 |
Number of pages | 4 |
Journal | Chemical Physics Letters |
Volume | 513 |
Issue number | 4-6 |
DOIs | |
Publication status | Published - 15 Sept 2011 |
Externally published | Yes |