Phosphatidic acid is a major phospholipid class in reproductive organs of arabidopsis thaliana

Ian Sofian Yunus; Amaury Cazenave-Gassiot; Yu Chi Liu; Ying Chen Lin; Markus R. Wenk; Yuki Nakamura

doi:10.1080/15592324.2015.1049790

Phosphatidic acid is a major phospholipid class in reproductive organs of arabidopsis thaliana

Ian Sofian Yunus, Amaury Cazenave-Gassiot, Yu Chi Liu, Ying Chen Lin, Markus R. Wenk, Yuki Nakamura^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Phospholipids are the crucial components of biological membranes and signal transduction. Among different tissues, flower phospholipids are one of the least characterized features of plant lipidome. Here, we report that floral reproductive organs of Arabidopsis thaliana contain high levels of phosphatidic acid (PA), a known lipid second messenger. By using floral homeotic mutants enriched with specific floral organs, lipidomics study showed increased levels of PA species in ap3-3 mutant with enriched pistils. Accompanied gene expression study for 7 diacylglycerol kinases and 11 PA phosphatases revealed distinct floral organ specificity, suggesting an active phosphorylation/dephosphorylation between PA and diacylglycerol in flowers. Our results suggest that PA is a major phospholipid class in floral reproductive organs of A. thaliana.

Original language	English
Journal	Plant Signaling and Behavior
Volume	10
Issue number	8
DOIs	https://doi.org/10.1080/15592324.2015.1049790
Publication status	Published - 2015
Externally published	Yes

Keywords

Arabidopsis thaliana
Floral reproductive organs
Glycerolipids
Homeotic mutants
Phosphatidic acid

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10.1080/15592324.2015.1049790

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title = "Phosphatidic acid is a major phospholipid class in reproductive organs of arabidopsis thaliana",

abstract = "Phospholipids are the crucial components of biological membranes and signal transduction. Among different tissues, flower phospholipids are one of the least characterized features of plant lipidome. Here, we report that floral reproductive organs of Arabidopsis thaliana contain high levels of phosphatidic acid (PA), a known lipid second messenger. By using floral homeotic mutants enriched with specific floral organs, lipidomics study showed increased levels of PA species in ap3-3 mutant with enriched pistils. Accompanied gene expression study for 7 diacylglycerol kinases and 11 PA phosphatases revealed distinct floral organ specificity, suggesting an active phosphorylation/dephosphorylation between PA and diacylglycerol in flowers. Our results suggest that PA is a major phospholipid class in floral reproductive organs of A. thaliana.",

keywords = "Arabidopsis thaliana, Floral reproductive organs, Glycerolipids, Homeotic mutants, Phosphatidic acid",

author = "Yunus, {Ian Sofian} and Amaury Cazenave-Gassiot and Liu, {Yu Chi} and Lin, {Ying Chen} and Wenk, {Markus R.} and Yuki Nakamura",

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doi = "10.1080/15592324.2015.1049790",

language = "English",

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journal = "Plant Signaling and Behavior",

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TY - JOUR

T1 - Phosphatidic acid is a major phospholipid class in reproductive organs of arabidopsis thaliana

AU - Yunus, Ian Sofian

AU - Cazenave-Gassiot, Amaury

AU - Liu, Yu Chi

AU - Lin, Ying Chen

AU - Wenk, Markus R.

AU - Nakamura, Yuki

PY - 2015

Y1 - 2015

N2 - Phospholipids are the crucial components of biological membranes and signal transduction. Among different tissues, flower phospholipids are one of the least characterized features of plant lipidome. Here, we report that floral reproductive organs of Arabidopsis thaliana contain high levels of phosphatidic acid (PA), a known lipid second messenger. By using floral homeotic mutants enriched with specific floral organs, lipidomics study showed increased levels of PA species in ap3-3 mutant with enriched pistils. Accompanied gene expression study for 7 diacylglycerol kinases and 11 PA phosphatases revealed distinct floral organ specificity, suggesting an active phosphorylation/dephosphorylation between PA and diacylglycerol in flowers. Our results suggest that PA is a major phospholipid class in floral reproductive organs of A. thaliana.

AB - Phospholipids are the crucial components of biological membranes and signal transduction. Among different tissues, flower phospholipids are one of the least characterized features of plant lipidome. Here, we report that floral reproductive organs of Arabidopsis thaliana contain high levels of phosphatidic acid (PA), a known lipid second messenger. By using floral homeotic mutants enriched with specific floral organs, lipidomics study showed increased levels of PA species in ap3-3 mutant with enriched pistils. Accompanied gene expression study for 7 diacylglycerol kinases and 11 PA phosphatases revealed distinct floral organ specificity, suggesting an active phosphorylation/dephosphorylation between PA and diacylglycerol in flowers. Our results suggest that PA is a major phospholipid class in floral reproductive organs of A. thaliana.

KW - Arabidopsis thaliana

KW - Floral reproductive organs

KW - Glycerolipids

KW - Homeotic mutants

KW - Phosphatidic acid

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Phosphatidic acid is a major phospholipid class in reproductive organs of arabidopsis thaliana

Abstract

Keywords

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