Phytoestrogen Cimicifugoside-Mediated Inhibition of Catecholamine Secretion by Blocking Nicotinic Acetylcholine Receptor in Bovine Adrenal Chromaffin Cells

Kyung Chul Woo, Yong Soo Park, Dong Jae Jun, Jeong Ok Lim, Woon Yi Baek, Byung Sun Suh, Kyong Tai Kim*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

We investigated the effect of the phytoestrogen cimicifugoside, one of the pharmacologically active ingredients of the medicinal plant Cimicifuga racemosa (black cohosh) that has been used to treat many kinds of neuronal and menopausal symptoms, such as arthritis, menopausal depression, and nerve pain. Cimicifugoside inhibited calcium increase induced by 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP), a nicotinic acetylcholine receptor (nAChR) agonist in bovine adrenal chromaffin cells with a half-maximal inhibitory concentration (IC50) of 18 ± 2 μM. In contrast, cimicifugoside did not affect the calcium increases evoked by high K +, veratridine, and bradykinin. The DMPP-induced sodium increase was also inhibited by cimicifugoside with an IC50 of 2 ± 0.3 μM, suggesting that the activity of nAChRs is inhibited by cimicifugoside. Cimicifugoside did not affect the KCl-induced secretion but markedly inhibited the DMPP-induced catecholamine secretion that was monitored by carbon-fiber amperometry in real time and high-performance liquid chromatography through electrochemical detection. The results suggest that cimicifugoside selectively inhibits nAChR-mediated response in bovine chromaffin cells.

Original languageEnglish
Pages (from-to)641-649
Number of pages9
JournalJournal of Pharmacology and Experimental Therapeutics
Volume309
Issue number2
DOIs
Publication statusPublished - May 2004
Externally publishedYes

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