PIP kinase Iγ is the major PI(4,5)P2 synthesizing enzyme at the synapse

Markus R. Wenk; Lorenzo Pellegrini; Vadim A. Klenchin; Gilbert Di Paolo; Sunghoe Chang; Laurie Daniell; Manabu Arioka; Thomas F. Martin; Pietro De Camilli

doi:10.1016/S0896-6273(01)00456-1

PIP kinase Iγ is the major PI(4,5)P₂ synthesizing enzyme at the synapse

Markus R. Wenk, Lorenzo Pellegrini, Vadim A. Klenchin, Gilbert Di Paolo, Sunghoe Chang, Laurie Daniell, Manabu Arioka, Thomas F. Martin, Pietro De Camilli^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

210 Citations (Scopus)

Abstract

Disruption of the presynaptically enriched polyphosphoinositide phosphatase synaptojanin 1 leads to an increase of clathrin-coated intermediates and of polymerized actin at endocytic zones of nerve terminals. These changes correlate with elevated levels of PI(4,5)P₂ in neurons. We report that phosphatidylinositol phosphate kinase type Iγ (PIPKIγ), a major brain PI(4)P 5-kinase, is concentrated at synapses. Synaptojanin 1 and PIPKIγ antagonize each other in the recruitment of clathrin coats to lipid membranes. Like synaptojanin 1 and other proteins involved in endocytosis, PIPKIγ undergoes stimulation-dependent dephosphorylation. These results implicate PIPKIγ in the synthesis of a PI(4,5)P₂ pool that acts as a positive regulator of clathrin coat recruitment and actin function at the synapse.

Original language	English
Pages (from-to)	79-88
Number of pages	10
Journal	Neuron
Volume	32
Issue number	1
DOIs	https://doi.org/10.1016/S0896-6273(01)00456-1
Publication status	Published - 2001
Externally published	Yes

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title = "PIP kinase Iγ is the major PI(4,5)P2 synthesizing enzyme at the synapse",

abstract = "Disruption of the presynaptically enriched polyphosphoinositide phosphatase synaptojanin 1 leads to an increase of clathrin-coated intermediates and of polymerized actin at endocytic zones of nerve terminals. These changes correlate with elevated levels of PI(4,5)P2 in neurons. We report that phosphatidylinositol phosphate kinase type Iγ (PIPKIγ), a major brain PI(4)P 5-kinase, is concentrated at synapses. Synaptojanin 1 and PIPKIγ antagonize each other in the recruitment of clathrin coats to lipid membranes. Like synaptojanin 1 and other proteins involved in endocytosis, PIPKIγ undergoes stimulation-dependent dephosphorylation. These results implicate PIPKIγ in the synthesis of a PI(4,5)P2 pool that acts as a positive regulator of clathrin coat recruitment and actin function at the synapse.",

author = "Wenk, {Markus R.} and Lorenzo Pellegrini and Klenchin, {Vadim A.} and {Di Paolo}, Gilbert and Sunghoe Chang and Laurie Daniell and Manabu Arioka and Martin, {Thomas F.} and {De Camilli}, Pietro",

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T1 - PIP kinase Iγ is the major PI(4,5)P2 synthesizing enzyme at the synapse

AU - Wenk, Markus R.

AU - Pellegrini, Lorenzo

AU - Klenchin, Vadim A.

AU - Di Paolo, Gilbert

AU - Chang, Sunghoe

AU - Daniell, Laurie

AU - Arioka, Manabu

AU - Martin, Thomas F.

AU - De Camilli, Pietro

PY - 2001

Y1 - 2001

N2 - Disruption of the presynaptically enriched polyphosphoinositide phosphatase synaptojanin 1 leads to an increase of clathrin-coated intermediates and of polymerized actin at endocytic zones of nerve terminals. These changes correlate with elevated levels of PI(4,5)P2 in neurons. We report that phosphatidylinositol phosphate kinase type Iγ (PIPKIγ), a major brain PI(4)P 5-kinase, is concentrated at synapses. Synaptojanin 1 and PIPKIγ antagonize each other in the recruitment of clathrin coats to lipid membranes. Like synaptojanin 1 and other proteins involved in endocytosis, PIPKIγ undergoes stimulation-dependent dephosphorylation. These results implicate PIPKIγ in the synthesis of a PI(4,5)P2 pool that acts as a positive regulator of clathrin coat recruitment and actin function at the synapse.

AB - Disruption of the presynaptically enriched polyphosphoinositide phosphatase synaptojanin 1 leads to an increase of clathrin-coated intermediates and of polymerized actin at endocytic zones of nerve terminals. These changes correlate with elevated levels of PI(4,5)P2 in neurons. We report that phosphatidylinositol phosphate kinase type Iγ (PIPKIγ), a major brain PI(4)P 5-kinase, is concentrated at synapses. Synaptojanin 1 and PIPKIγ antagonize each other in the recruitment of clathrin coats to lipid membranes. Like synaptojanin 1 and other proteins involved in endocytosis, PIPKIγ undergoes stimulation-dependent dephosphorylation. These results implicate PIPKIγ in the synthesis of a PI(4,5)P2 pool that acts as a positive regulator of clathrin coat recruitment and actin function at the synapse.

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VL - 32

SP - 79

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JO - Neuron

JF - Neuron

IS - 1

ER -

PIP kinase Iγ is the major PI(4,5)P₂ synthesizing enzyme at the synapse

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