Pluripotency activity of nanog requires biochemical stabilization by variant histone protein H2A.Z

Jiaxu Wang; Mengran Qiao; Qianqian He; Ronghua Shi; Sharon Jia Hui Loh; Lawrence W. Stanton; Mian Wu

doi:10.1002/stem.2011

Pluripotency activity of nanog requires biochemical stabilization by variant histone protein H2A.Z

Jiaxu Wang, Mengran Qiao, Qianqian He, Ronghua Shi, Sharon Jia Hui Loh, Lawrence W. Stanton^*, Mian Wu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The variant histone protein H2A.Z plays a critical role in early development. Likewise, Nanog, a master regulator of embryonic stem cells (ESCs), is essential for proper development in early embryogenesis. In this study, we establish that these two factors work together to maintain pluripotency. It is shown that H2A.Z influences the protein level of Nanog through the ubiquitin-proteasome pathway. Knockdown of H2A.Z causes differentiation of mouse ESCs and disrupts the reprogramming of somatic cells, which can be partially rescued by overexpression of Nanog. We conclude that the H2A.Z-Nanog partnership is involved in ESC pluripotency and reprogramming of somatic cells.

Original language	English
Pages (from-to)	2126-2134
Number of pages	9
Journal	Stem Cells
Volume	33
Issue number	7
DOIs	https://doi.org/10.1002/stem.2011
Publication status	Published - 1 Jul 2015
Externally published	Yes

Keywords

Differentiation of stem cells
H2A.Z
Induced pluripotent stem cells
Ubiquitination of Nanog

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10.1002/stem.2011

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abstract = "The variant histone protein H2A.Z plays a critical role in early development. Likewise, Nanog, a master regulator of embryonic stem cells (ESCs), is essential for proper development in early embryogenesis. In this study, we establish that these two factors work together to maintain pluripotency. It is shown that H2A.Z influences the protein level of Nanog through the ubiquitin-proteasome pathway. Knockdown of H2A.Z causes differentiation of mouse ESCs and disrupts the reprogramming of somatic cells, which can be partially rescued by overexpression of Nanog. We conclude that the H2A.Z-Nanog partnership is involved in ESC pluripotency and reprogramming of somatic cells.",

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AU - Wang, Jiaxu

AU - Qiao, Mengran

AU - He, Qianqian

AU - Shi, Ronghua

AU - Loh, Sharon Jia Hui

AU - Stanton, Lawrence W.

AU - Wu, Mian

PY - 2015/7/1

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Pluripotency activity of nanog requires biochemical stabilization by variant histone protein H2A.Z

Abstract

Keywords

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