Abstract
Plasmids were constructed to supply cII-coded protein for activation of the phage promoter p1. Using a fusion which expresses lacZ from p1, we can accurately follow activation of p1 without having to assay int activity in vivo. A large excess of cII protein compared to a normal lytic infection stimulates lacZ expression about 10-fold over the basal level. The int-c226 constitutive allele of p1 is not further activated by cII even though its level of lacZ expression is less than the maximal cII-activated expression from wild type p1. A himA-deleted strain also shows activation, demonstrating that there is no absolute requirement for the himA gene product for ell-stimulated transcription at pI.
Original language | English |
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Pages (from-to) | 303-311 |
Number of pages | 9 |
Journal | Gene |
Volume | 19 |
Issue number | 3 |
DOIs | |
Publication status | Published - Oct 1982 |
Externally published | Yes |
Keywords
- E. coli phage
- Gene cloning
- deletions
- hydroxylamine mutagenesis of plasmids
- recombinant DNA
- trp-lac fusions