Probing cII and himA action at the integrase promoter p1 of bacteriophage lambda

Michael Benedik*, Desmond Mascarenhas, Allan Campbell

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Plasmids were constructed to supply cII-coded protein for activation of the phage promoter p1. Using a fusion which expresses lacZ from p1, we can accurately follow activation of p1 without having to assay int activity in vivo. A large excess of cII protein compared to a normal lytic infection stimulates lacZ expression about 10-fold over the basal level. The int-c226 constitutive allele of p1 is not further activated by cII even though its level of lacZ expression is less than the maximal cII-activated expression from wild type p1. A himA-deleted strain also shows activation, demonstrating that there is no absolute requirement for the himA gene product for ell-stimulated transcription at pI.

Original languageEnglish
Pages (from-to)303-311
Number of pages9
JournalGene
Volume19
Issue number3
DOIs
Publication statusPublished - Oct 1982
Externally publishedYes

Keywords

  • E. coli phage
  • Gene cloning
  • deletions
  • hydroxylamine mutagenesis of plasmids
  • recombinant DNA
  • trp-lac fusions

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