TY - JOUR
T1 - Psychrophilic enzymes
T2 - A thermodynamic challenge
AU - Gerday, Charles
AU - Aittaleb, Mohamed
AU - Arpigny, Jean Louis
AU - Baise, Etienne
AU - Chessa, Jean Pierre
AU - Garsoux, Geneviève
AU - Petrescu, Ioan
AU - Feller, Georges
PY - 1997/10/17
Y1 - 1997/10/17
N2 - Psychrophilic microorganisms, hosts of permanently cold habitats, produce enzymes which are adapted to work at low temperatures. When compared to their mesophilic counterparts, these enzymes display a higher catalytic efficiency over a temperature range of roughly 0-30°C and a high thermosensitivity. The molecular characteristics of cold enzymes originating from Antarctic bacteria have been approached through protein modelling and X-ray crystallography. The deduced three-dimensional structures of cold α-amylase, β-lactamase, lipase and subtilisin have been compared to their mesophilic homologs. It appears that the molecular adaptation resides in a weakening of the intramolecular interactions, and in some cases in an increase of the interaction with the solvent, leading to more flexible molecular edifices capable of performing catalysis at a lower energy cost.
AB - Psychrophilic microorganisms, hosts of permanently cold habitats, produce enzymes which are adapted to work at low temperatures. When compared to their mesophilic counterparts, these enzymes display a higher catalytic efficiency over a temperature range of roughly 0-30°C and a high thermosensitivity. The molecular characteristics of cold enzymes originating from Antarctic bacteria have been approached through protein modelling and X-ray crystallography. The deduced three-dimensional structures of cold α-amylase, β-lactamase, lipase and subtilisin have been compared to their mesophilic homologs. It appears that the molecular adaptation resides in a weakening of the intramolecular interactions, and in some cases in an increase of the interaction with the solvent, leading to more flexible molecular edifices capable of performing catalysis at a lower energy cost.
KW - Adaptation to cold
KW - Antarctic microorganism
KW - Psychrophile
UR - http://www.scopus.com/inward/record.url?scp=0030699980&partnerID=8YFLogxK
U2 - 10.1016/S0167-4838(97)00093-9
DO - 10.1016/S0167-4838(97)00093-9
M3 - Review article
C2 - 9392521
AN - SCOPUS:0030699980
SN - 0167-4838
VL - 1342
SP - 119
EP - 131
JO - Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
JF - Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
IS - 2
ER -