Rapid isolation of any known genes from whole cells of yeast by PCR

Dindial Ramotar

doi:10.1007/BF00935491

Rapid isolation of any known genes from whole cells of yeast by PCR

Dindial Ramotar^*

^*Corresponding author for this work

Université Laval

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

A short protocol was developed that allows the rapid isolation of any known Saccharomyces cerevisiae gene. Two known genes, APN1 and IMP2, were isolated directly from whole cells of yeast using polymerase chain reaction, without the need for purified template genomic DNA.

Original language	English
Pages (from-to)	185-187
Number of pages	3
Journal	Molecular and Cellular Biochemistry
Volume	145
Issue number	2
DOIs	https://doi.org/10.1007/BF00935491
Publication status	Published - Apr 1995
Externally published	Yes

Keywords

gene
method
polymerase chain reaction
yeast

Access to Document

10.1007/BF00935491

Cite this

@article{c998256832a54a1681bea4abe7b04b21,

title = "Rapid isolation of any known genes from whole cells of yeast by PCR",

abstract = "A short protocol was developed that allows the rapid isolation of any known Saccharomyces cerevisiae gene. Two known genes, APN1 and IMP2, were isolated directly from whole cells of yeast using polymerase chain reaction, without the need for purified template genomic DNA.",

keywords = "gene, method, polymerase chain reaction, yeast",

author = "Dindial Ramotar",

year = "1995",

month = apr,

doi = "10.1007/BF00935491",

language = "English",

volume = "145",

pages = "185--187",

journal = "Molecular and Cellular Biochemistry",

issn = "0300-8177",

publisher = "Springer",

number = "2",

}

TY - JOUR

T1 - Rapid isolation of any known genes from whole cells of yeast by PCR

AU - Ramotar, Dindial

PY - 1995/4

Y1 - 1995/4

N2 - A short protocol was developed that allows the rapid isolation of any known Saccharomyces cerevisiae gene. Two known genes, APN1 and IMP2, were isolated directly from whole cells of yeast using polymerase chain reaction, without the need for purified template genomic DNA.

AB - A short protocol was developed that allows the rapid isolation of any known Saccharomyces cerevisiae gene. Two known genes, APN1 and IMP2, were isolated directly from whole cells of yeast using polymerase chain reaction, without the need for purified template genomic DNA.

KW - gene

KW - method

KW - polymerase chain reaction

KW - yeast

UR - http://www.scopus.com/inward/record.url?scp=0029061754&partnerID=8YFLogxK

U2 - 10.1007/BF00935491

DO - 10.1007/BF00935491

M3 - Article

C2 - 7675038

AN - SCOPUS:0029061754

SN - 0300-8177

VL - 145

SP - 185

EP - 187

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

IS - 2

ER -

Rapid isolation of any known genes from whole cells of yeast by PCR

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this