TY - JOUR
T1 - Retroregulation
T2 - Control of integrase expression by the b2 region of bacteriophages λ and 434
AU - Mascarenhas, D.
AU - Trueheart, J.
AU - Benedik, M.
AU - Campbell, A.
PY - 1983/1/15
Y1 - 1983/1/15
N2 - Genetic fusions constructed by a combination of in vivo and in vitro techniques have been used to investigate the cis-regulatory role of a 250-base-pair segment of b2 DNA in the expression of int transcripts originating from three different promoters PI, PL and PTRP. It has been established that (a) the PI and PTRP transcripts, which produce functional int protein, are efficiently terminated by the b2 segment, (b) in the same test system, the PL transcript, which does not result in functional integrase, is not terminated by the b2 segment, (c) this b2 (sib) effect does not depend on genes lying between int and N on the phage genome, (d) the sib effect is also observed with the b2 DNA of phage 434, which resembles that of λ between -197 and att but diverges radically from it to the left of base -197.
AB - Genetic fusions constructed by a combination of in vivo and in vitro techniques have been used to investigate the cis-regulatory role of a 250-base-pair segment of b2 DNA in the expression of int transcripts originating from three different promoters PI, PL and PTRP. It has been established that (a) the PI and PTRP transcripts, which produce functional int protein, are efficiently terminated by the b2 segment, (b) in the same test system, the PL transcript, which does not result in functional integrase, is not terminated by the b2 segment, (c) this b2 (sib) effect does not depend on genes lying between int and N on the phage genome, (d) the sib effect is also observed with the b2 DNA of phage 434, which resembles that of λ between -197 and att but diverges radically from it to the left of base -197.
UR - http://www.scopus.com/inward/record.url?scp=0020672091&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(83)90293-3
DO - 10.1016/0042-6822(83)90293-3
M3 - Article
C2 - 6297148
AN - SCOPUS:0020672091
SN - 0042-6822
VL - 124
SP - 100
EP - 108
JO - Virology
JF - Virology
IS - 1
ER -