RNA polymerase II degradation in response to rapamycin is not mediated through ubiquitylation

Nathalie Jouvet; Jeremie Poschmann; Julie Douville; Rim Marrakchi; Dindial Ramotar

doi:10.1016/j.bbrc.2011.08.079

RNA polymerase II degradation in response to rapamycin is not mediated through ubiquitylation

Nathalie Jouvet, Jeremie Poschmann, Julie Douville, Rim Marrakchi, Dindial Ramotar^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

In Saccharomyces cerevisiae, the immunosuppressor rapamycin engenders the degradation of excessive RNA polymerase II leading to growth arrest but the regulation of this process is not known yet. Here, we show that this mechanism is dependent on the peptidyl prolyl cis/. trans isomerase Rrd1. Strikingly this degradation is independent of RNA polymerase II polyubiquitylation and does not require the elongation factor Elc1. Our data reveal that there are at least two alternative pathways to degrade RNA polymerase II that depend on different type of stresses.

Original language	English
Pages (from-to)	248-253
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	413
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2011.08.079
Publication status	Published - 23 Sept 2011
Externally published	Yes

Keywords

Peptidyl prolyl isomerase
RNA polymerase II degradation
Rapamycin
Rrd1
Ubiquitylation

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10.1016/j.bbrc.2011.08.079

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title = "RNA polymerase II degradation in response to rapamycin is not mediated through ubiquitylation",

abstract = "In Saccharomyces cerevisiae, the immunosuppressor rapamycin engenders the degradation of excessive RNA polymerase II leading to growth arrest but the regulation of this process is not known yet. Here, we show that this mechanism is dependent on the peptidyl prolyl cis/. trans isomerase Rrd1. Strikingly this degradation is independent of RNA polymerase II polyubiquitylation and does not require the elongation factor Elc1. Our data reveal that there are at least two alternative pathways to degrade RNA polymerase II that depend on different type of stresses.",

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AU - Jouvet, Nathalie

AU - Poschmann, Jeremie

AU - Douville, Julie

AU - Marrakchi, Rim

AU - Ramotar, Dindial

PY - 2011/9/23

Y1 - 2011/9/23

N2 - In Saccharomyces cerevisiae, the immunosuppressor rapamycin engenders the degradation of excessive RNA polymerase II leading to growth arrest but the regulation of this process is not known yet. Here, we show that this mechanism is dependent on the peptidyl prolyl cis/. trans isomerase Rrd1. Strikingly this degradation is independent of RNA polymerase II polyubiquitylation and does not require the elongation factor Elc1. Our data reveal that there are at least two alternative pathways to degrade RNA polymerase II that depend on different type of stresses.

AB - In Saccharomyces cerevisiae, the immunosuppressor rapamycin engenders the degradation of excessive RNA polymerase II leading to growth arrest but the regulation of this process is not known yet. Here, we show that this mechanism is dependent on the peptidyl prolyl cis/. trans isomerase Rrd1. Strikingly this degradation is independent of RNA polymerase II polyubiquitylation and does not require the elongation factor Elc1. Our data reveal that there are at least two alternative pathways to degrade RNA polymerase II that depend on different type of stresses.

KW - Peptidyl prolyl isomerase

KW - RNA polymerase II degradation

KW - Rapamycin

KW - Rrd1

KW - Ubiquitylation

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EP - 253

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

RNA polymerase II degradation in response to rapamycin is not mediated through ubiquitylation

Abstract

Keywords

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