Sphingolipid Analysis in Clinical Research

Bo Burla, Sneha Muralidharan, Markus R. Wenk, Federico Torta*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

29 Citations (Scopus)

Abstract

Sphingolipids are the most diverse class of lipids due to the numerous variations in their structural components. This diversity is also reflected in their extremely different functions. Sphingolipids are not only constituents of cell membranes but have also emerged as key signaling molecules involved in a variety of cellular functions, such as cell growth and differentiation, proliferation, and apoptotic cell death. Lipidomic analyses in clinical research have identified pathways and products of sphingolipid metabolism that are altered in several human pathologies. In this article, we describe how to properly design a lipidomic experiment in clinical research, how to handle plasma and serum samples for this purpose, and how to measure sphingolipids using liquid chromatography-mass spectrometry.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages135-162
Number of pages28
DOIs
Publication statusPublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1730
ISSN (Print)1064-3745

Keywords

  • Ceramide
  • Clinical mass spectrometry
  • Glucosylceramide
  • Lipidomics
  • Mass spectrometry
  • Quality control
  • Sphingolipidomics
  • Sphingolipids
  • Sphingomyelin
  • Sphingosine-1-phosphate

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