Stabilization of folding intermediate states from alkaline induced unfolded state of bovine serum fetuin in trifluoroethanol and acetonitrile

Basir Ahmad; Zeyaul Islam; Ankita Varshney; Rizwan Hasan Khan

doi:10.2174/092986610791112774

Stabilization of folding intermediate states from alkaline induced unfolded state of bovine serum fetuin in trifluoroethanol and acetonitrile

Basir Ahmad, Zeyaul Islam, Ankita Varshney, Rizwan Hasan Khan

Aligarh Muslim University

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The conformation of bovine serum fetuin (BSF) was examined over the pH 7.0-12.9 regions by circular dichro-ism, intrinsic fluorescence and ANS binding. We observed that at higher pH, BSF exists in alkaline unfolded state. Our results provided evidence that correlates simultaneous formation of secondary structure followed by accumulation of hy-drophobic clusters.

Original language	English
Pages (from-to)	660-666
Number of pages	7
Journal	Protein and Peptide Letters
Volume	17
Issue number	5
DOIs	https://doi.org/10.2174/092986610791112774
Publication status	Published - 2010
Externally published	Yes

Keywords

Acetonitrile
Alkali denaturation
Bovine serum fetuin
Circular dichroism
Trifluoroethanol

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abstract = "The conformation of bovine serum fetuin (BSF) was examined over the pH 7.0-12.9 regions by circular dichro-ism, intrinsic fluorescence and ANS binding. We observed that at higher pH, BSF exists in alkaline unfolded state. Our results provided evidence that correlates simultaneous formation of secondary structure followed by accumulation of hy-drophobic clusters.",

keywords = "Acetonitrile, Alkali denaturation, Bovine serum fetuin, Circular dichroism, Trifluoroethanol",

author = "Basir Ahmad and Zeyaul Islam and Ankita Varshney and Khan, {Rizwan Hasan}",

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pages = "660--666",

journal = "Protein and Peptide Letters",

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T1 - Stabilization of folding intermediate states from alkaline induced unfolded state of bovine serum fetuin in trifluoroethanol and acetonitrile

AU - Ahmad, Basir

AU - Islam, Zeyaul

AU - Varshney, Ankita

AU - Khan, Rizwan Hasan

PY - 2010

Y1 - 2010

N2 - The conformation of bovine serum fetuin (BSF) was examined over the pH 7.0-12.9 regions by circular dichro-ism, intrinsic fluorescence and ANS binding. We observed that at higher pH, BSF exists in alkaline unfolded state. Our results provided evidence that correlates simultaneous formation of secondary structure followed by accumulation of hy-drophobic clusters.

AB - The conformation of bovine serum fetuin (BSF) was examined over the pH 7.0-12.9 regions by circular dichro-ism, intrinsic fluorescence and ANS binding. We observed that at higher pH, BSF exists in alkaline unfolded state. Our results provided evidence that correlates simultaneous formation of secondary structure followed by accumulation of hy-drophobic clusters.

KW - Acetonitrile

KW - Alkali denaturation

KW - Bovine serum fetuin

KW - Circular dichroism

KW - Trifluoroethanol

UR - http://www.scopus.com/inward/record.url?scp=77952004183&partnerID=8YFLogxK

U2 - 10.2174/092986610791112774

DO - 10.2174/092986610791112774

M3 - Article

C2 - 19995341

AN - SCOPUS:77952004183

SN - 0929-8665

VL - 17

SP - 660

EP - 666

JO - Protein and Peptide Letters

JF - Protein and Peptide Letters

IS - 5

ER -

Stabilization of folding intermediate states from alkaline induced unfolded state of bovine serum fetuin in trifluoroethanol and acetonitrile

Abstract

Keywords

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