TY - JOUR
T1 - Studies into the interaction of a ferrocene-conjugates of Gly-Gly-Arg-Tyr with papain
T2 - AC voltammetry, impedance spectroscopy and surface plasmon resonance studies
AU - Tharamani, Chikka N.
AU - Mahmoud, Khaled A.
AU - Vasanthakumar, Ganga Ramu
AU - Kraatz, Heinz Bernard
PY - 2009/3/28
Y1 - 2009/3/28
N2 - The interaction of papain with peptide immobilized on the Au surface altered the interfacial electron transfer resistant, charge transfer resistance (RCT), by preventing the redox species approaching the electrode was characterized by electrochemical impedance spectroscopy (EIS), AC voltammetry, cyclic voltammetry and surface plasmon resonance techniques (SPR). From cyclic voltammetry a potential shift of 25-30 mV was observed after the addition of papain, with decrease in current and the impedance results showed that RCT increased with increasing concentration of papain indicating the interaction of papain with the immobilized film. This change in kinetics was also observed in SPR. The interaction was further confirmed by reflection-absorption infrared spectroscopy (RAIR).
AB - The interaction of papain with peptide immobilized on the Au surface altered the interfacial electron transfer resistant, charge transfer resistance (RCT), by preventing the redox species approaching the electrode was characterized by electrochemical impedance spectroscopy (EIS), AC voltammetry, cyclic voltammetry and surface plasmon resonance techniques (SPR). From cyclic voltammetry a potential shift of 25-30 mV was observed after the addition of papain, with decrease in current and the impedance results showed that RCT increased with increasing concentration of papain indicating the interaction of papain with the immobilized film. This change in kinetics was also observed in SPR. The interaction was further confirmed by reflection-absorption infrared spectroscopy (RAIR).
KW - Electrochemical impedance spectroscopy
KW - Papain
KW - Peptides
KW - SPR
KW - Sensors
UR - http://www.scopus.com/inward/record.url?scp=60549092246&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2008.11.050
DO - 10.1016/j.snb.2008.11.050
M3 - Article
AN - SCOPUS:60549092246
SN - 0925-4005
VL - 137
SP - 253
EP - 258
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
IS - 1
ER -