Superresolution microscopy of the nuclear envelope and associated proteins

Wei Xie; Henning F. Horn; Graham D. Wright

doi:10.1007/978-1-4939-3530-7_4

Superresolution microscopy of the nuclear envelope and associated proteins

Wei Xie, Henning F. Horn, Graham D. Wright

Biological and Biomedical Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

6 Citations (Scopus)

Abstract

Superresolution microscopy is undoubtedly one of the most exciting technologies since the invention of the optical microscope. Capable of nanometer-scale resolution to surpass the diffraction limit and coupled with the versatile labeling techniques available, it is revolutionizing the study of cell biology. Our understanding of the nucleus, the genetic and architectural center of the cell, has gained great advancements through the application of various superresolution microscopy techniques. This chapter describes detailed procedures of multichannel superresolution imaging of the mammalian nucleus, using structured illumination microscopy and single-molecule localization microscopy.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	83-97
Number of pages	15
DOIs	https://doi.org/10.1007/978-1-4939-3530-7_4
Publication status	Published - 2016

Publication series

Name	Methods in Molecular Biology
Volume	1411
ISSN (Print)	1064-3745

Keywords

Linker of the nucleoskeleton and cytoskeleton (LINC) complex
Nuclear envelope (NE)
Nuclear lamins
Nuclear pore complex (NPC)
Photoactivated localization microscopy (PALM)
Singlemolecule localization microscopy (SMLM)
Stochastic optical reconstruction microscopy (STORM)
Structured illumination microscopy (SIM)
Superresolution microscopy (SRM)
Synaptonemal complex

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10.1007/978-1-4939-3530-7_4

Cite this

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title = "Superresolution microscopy of the nuclear envelope and associated proteins",

abstract = "Superresolution microscopy is undoubtedly one of the most exciting technologies since the invention of the optical microscope. Capable of nanometer-scale resolution to surpass the diffraction limit and coupled with the versatile labeling techniques available, it is revolutionizing the study of cell biology. Our understanding of the nucleus, the genetic and architectural center of the cell, has gained great advancements through the application of various superresolution microscopy techniques. This chapter describes detailed procedures of multichannel superresolution imaging of the mammalian nucleus, using structured illumination microscopy and single-molecule localization microscopy.",

keywords = "Linker of the nucleoskeleton and cytoskeleton (LINC) complex, Nuclear envelope (NE), Nuclear lamins, Nuclear pore complex (NPC), Photoactivated localization microscopy (PALM), Singlemolecule localization microscopy (SMLM), Stochastic optical reconstruction microscopy (STORM), Structured illumination microscopy (SIM), Superresolution microscopy (SRM), Synaptonemal complex",

author = "Wei Xie and Horn, {Henning F.} and Wright, {Graham D.}",

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year = "2016",

doi = "10.1007/978-1-4939-3530-7_4",

language = "English",

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publisher = "Humana Press Inc.",

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T1 - Superresolution microscopy of the nuclear envelope and associated proteins

AU - Xie, Wei

AU - Horn, Henning F.

AU - Wright, Graham D.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2016.

PY - 2016

Y1 - 2016

N2 - Superresolution microscopy is undoubtedly one of the most exciting technologies since the invention of the optical microscope. Capable of nanometer-scale resolution to surpass the diffraction limit and coupled with the versatile labeling techniques available, it is revolutionizing the study of cell biology. Our understanding of the nucleus, the genetic and architectural center of the cell, has gained great advancements through the application of various superresolution microscopy techniques. This chapter describes detailed procedures of multichannel superresolution imaging of the mammalian nucleus, using structured illumination microscopy and single-molecule localization microscopy.

AB - Superresolution microscopy is undoubtedly one of the most exciting technologies since the invention of the optical microscope. Capable of nanometer-scale resolution to surpass the diffraction limit and coupled with the versatile labeling techniques available, it is revolutionizing the study of cell biology. Our understanding of the nucleus, the genetic and architectural center of the cell, has gained great advancements through the application of various superresolution microscopy techniques. This chapter describes detailed procedures of multichannel superresolution imaging of the mammalian nucleus, using structured illumination microscopy and single-molecule localization microscopy.

KW - Linker of the nucleoskeleton and cytoskeleton (LINC) complex

KW - Nuclear envelope (NE)

KW - Nuclear lamins

KW - Nuclear pore complex (NPC)

KW - Photoactivated localization microscopy (PALM)

KW - Singlemolecule localization microscopy (SMLM)

KW - Stochastic optical reconstruction microscopy (STORM)

KW - Structured illumination microscopy (SIM)

KW - Superresolution microscopy (SRM)

KW - Synaptonemal complex

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DO - 10.1007/978-1-4939-3530-7_4

M3 - Chapter

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AN - SCOPUS:84965095467

T3 - Methods in Molecular Biology

SP - 83

EP - 97

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Superresolution microscopy of the nuclear envelope and associated proteins

Abstract

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Keywords

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