Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7

Robert R. Riddle; Richard C. Willson; Michael J. Benedik

doi:10.1023/A:1005402209276

Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7

Robert R. Riddle, Richard C. Willson, Michael J. Benedik^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Mutants of helper phage M13 KO7 were isolated whose survival was up to 300-fold greater than the wild type after 30 min at 95°C, and had increased solvent tolerance (up to 200-fold in 75% v/v N,N-dimethylformamide). These mutants retained their ability to package pIII-fusion display phagemids with normal efficiency and therefore will be useful in the isolation of stable protein variants by phage display.

Original language	English
Pages (from-to)	87-90
Number of pages	4
Journal	Biotechnology Letters
Volume	21
Issue number	1
DOIs	https://doi.org/10.1023/A:1005402209276
Publication status	Published - 1999
Externally published	Yes

Keywords

M13
Phage display
Solvent tolerance
Temperature resistance

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10.1023/A:1005402209276

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abstract = "Mutants of helper phage M13 KO7 were isolated whose survival was up to 300-fold greater than the wild type after 30 min at 95°C, and had increased solvent tolerance (up to 200-fold in 75% v/v N,N-dimethylformamide). These mutants retained their ability to package pIII-fusion display phagemids with normal efficiency and therefore will be useful in the isolation of stable protein variants by phage display.",

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AU - Willson, Richard C.

AU - Benedik, Michael J.

PY - 1999

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AB - Mutants of helper phage M13 KO7 were isolated whose survival was up to 300-fold greater than the wild type after 30 min at 95°C, and had increased solvent tolerance (up to 200-fold in 75% v/v N,N-dimethylformamide). These mutants retained their ability to package pIII-fusion display phagemids with normal efficiency and therefore will be useful in the isolation of stable protein variants by phage display.

KW - M13

KW - Phage display

KW - Solvent tolerance

KW - Temperature resistance

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DO - 10.1023/A:1005402209276

M3 - Article

AN - SCOPUS:0032922362

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IS - 1

ER -

Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7

Abstract

Keywords

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