The metalloprotease gene of Serratia marcescens strain SM6

Sharon C. Braunagel, Michael J. Benedik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Utilizing the DNA sequence of the metalloprotease from Serratia strain E-15, we isolated and sequenced the homologous gene from Serratia strain SM6. These two genes are similar at both the DNA and protein sequence level. Expression of the protease gene in Escherichia coli was achieved by use of the lac promoter. This resulted in the production and excretion of an immunologically detectable but inactive protein of slightly higher molecular weight than that from Serratia. We introduced the cloned gene into previously described protease mutants. The observed pattern of protease expression suggested that these mutations fall into three classes.

Original languageEnglish
Pages (from-to)446-451
Number of pages6
JournalMolecular Genetics and Genomics
Volume222
Issue number2-3
DOIs
Publication statusPublished - Jul 1990
Externally publishedYes

Keywords

  • Enterobacteriaceae
  • Extracellular proteins
  • Gene cloning
  • Protease
  • Secretion

Fingerprint

Dive into the research topics of 'The metalloprotease gene of Serratia marcescens strain SM6'. Together they form a unique fingerprint.

Cite this