TY - JOUR
T1 - Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation
AU - Brandenberger, Ralph
AU - Wei, Henry
AU - Zhang, Sally
AU - Lei, Shirley
AU - Murage, Jaji
AU - Fisk, Gregory J.
AU - Li, Yan
AU - Xu, Chunhui
AU - Fang, Rixun
AU - Guegler, Karl
AU - Rao, Mahendra S.
AU - Mandalam, Ramumkar
AU - Lebkowski, Jane
AU - Stanton, Lawrence W.
PY - 2004/6
Y1 - 2004/6
N2 - Human embryonic stem (hES) cells hold promise for generating an unlimited supply of cells for replacement therapies. To characterize hES cells at the molecular level, we obtained 148,453 expressed sequence tags (ESTs) from undifferentiated hES cells and three differentiated derivative subpopulations. Over 32,000 different transcripts expressed in hES cells were identified, of which more than 16,000 do not match closely any gene in the UniGene public database. Queries to this EST database revealed 532 significantly upregulated and 140 significantly downregulated genes in undifferentiated hES cells. These data highlight changes in the transcriptional network that occur when hES cells differentiate. Among the differentially regulated genes are several components of signaling pathways and transcriptional regulators that likely play key roles in hES cell growth and differentiation. The genomic data presented here may facilitate the derivation of clinically useful cell types from hES cells.
AB - Human embryonic stem (hES) cells hold promise for generating an unlimited supply of cells for replacement therapies. To characterize hES cells at the molecular level, we obtained 148,453 expressed sequence tags (ESTs) from undifferentiated hES cells and three differentiated derivative subpopulations. Over 32,000 different transcripts expressed in hES cells were identified, of which more than 16,000 do not match closely any gene in the UniGene public database. Queries to this EST database revealed 532 significantly upregulated and 140 significantly downregulated genes in undifferentiated hES cells. These data highlight changes in the transcriptional network that occur when hES cells differentiate. Among the differentially regulated genes are several components of signaling pathways and transcriptional regulators that likely play key roles in hES cell growth and differentiation. The genomic data presented here may facilitate the derivation of clinically useful cell types from hES cells.
UR - http://www.scopus.com/inward/record.url?scp=2542601547&partnerID=8YFLogxK
U2 - 10.1038/nbt971
DO - 10.1038/nbt971
M3 - Article
C2 - 15146197
AN - SCOPUS:2542601547
SN - 1087-0156
VL - 22
SP - 707
EP - 716
JO - Nature Biotechnology
JF - Nature Biotechnology
IS - 6
ER -