Uptake Assays to Monitor Anthracyclines Entry into Mammalian Cells

Nicolas Brosseau; Emil Andreev; Dindial Ramotar

doi:10.21769/BioProtoc.2555

Uptake Assays to Monitor Anthracyclines Entry into Mammalian Cells

Nicolas Brosseau, Emil Andreev, Dindial Ramotar

Universite du Quebec a Montreal (UQAM)

Research output: Contribution to journal › Article › peer-review

Abstract

Anthracyclines, such as doxorubicin and daunorubicin, are DNA damaging agents that autofluoresce and can be readily detected in cells. Herein, we developed suitable assays to quantify and localize daunorubicin in mammalian cells. These assays can be exploited to identify components that are involved in the uptake of anthracyclines.

Original language	English
Article number	e2555
Number of pages	22
Journal	Bio-protocol
Volume	7
Issue number	18
DOIs	https://doi.org/10.21769/BioProtoc.2555
Publication status	Published - 20 Sept 2017
Externally published	Yes

Keywords

Anthracyclines
Autofluorescent drugs
Epifluorescence microscopy
FACS analysis
Fluoroskan reader
Influx transporters

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10.21769/BioProtoc.2555

Cite this

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abstract = "Anthracyclines, such as doxorubicin and daunorubicin, are DNA damaging agents that autofluoresce and can be readily detected in cells. Herein, we developed suitable assays to quantify and localize daunorubicin in mammalian cells. These assays can be exploited to identify components that are involved in the uptake of anthracyclines.",

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KW - Autofluorescent drugs

KW - Epifluorescence microscopy

KW - FACS analysis

KW - Fluoroskan reader

KW - Influx transporters

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Uptake Assays to Monitor Anthracyclines Entry into Mammalian Cells

Abstract

Keywords

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